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Home / Proteins & Peptide

JARID1C(KDM5C), FLAG-tag

SKU : BHP18200338

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Mfr.No.	50112
Immunogen Region	2-1560(end)
Tag	N-terminal FLAG-tag
Description	Human JARID1C, also known as SMCX (Smcy homolog, X-linked), KDM5C, or XE169, GenBank Accession No. NM_004187, full length, a.a. 2-1560(end), with N-terminal FLAG- and His-tags, MW= 181 kDa, expressed in Sf9 cells via a baculovirus expression system. Host cell line: Sf9 cells Specific activity: 0.078 pmole/min/ug
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ug/ml FLAG peptide
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 ul reaction mix containing assay buffer with 20 mM HEPES (pH 7.4), 50 mM NaCl 500 uM alpha-ketoglutarate, 25 uM iron, 2 mM ascorbic acid, 0.01% Tween-20, 0.5 uM biotinylated peptide substrate, and JARID1C (50 - 200 ng) added to the wells. Add antibody against demethylated K4 peptide. Incubate for 30 min, then Streptavidin-conjugated secondary antibody followed by Alpha Screening detection. Avoid freeze/thaw cycles.
GenBank	NM_004187
Mw(kda)	181 kDa

Synonyms	SMCX, Smcy homolog, X-linked, KDM5C, XE169
Uniprot	P41229

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Xu, J., et al. (2008). PLoS One 3(7):e2553 2. Iwase, S., et al. (2007). Cell 128(6):1077-88. 3. Jenson, L.R., et al. (2005). Am. J. Hum. Genet. 76(2):227-36.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200338	50112	20 ug	$522.50		Add to Cart

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