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Home / Proteins & Peptide

Jak2 (JH1, JH2 domain), His-GST-tags

SKU : BHP18200248

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Mfr.No.	40451
Immunogen Region	532-1132
Tag	N-terminal His-GST-tags
Description	Human Jak2 (GenBank Accession No. NM_004972), containing the catalytic and the pseudo kinase domain (a.a. 532-1132) with N-terminal His-GST-tag, MW=98 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 8 pmol/min/ug
Formulation	45 mM Tris-HCl, pH 8.0, 300 mM NaCl, 2.4 mM KCl, 0.04% Tween-20, 3 mM DTT, 10% glycerol and 250 mM Imidazole
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme reaction is conducted in a buffer containing 50 mM HEPES, pH 7.5, 10 mM MgCl₂, 1 mM EGTA, 200 uM ATP, 0.01% Brij-35, 2 uM substrate (Tyr Peptide 6, Z-lyte kinase assay kit from Invitrogen), and 0-100 ng enzyme at 37°C for 1 hour. Avoid freeze/thaw cycles.
GenBank	NM_004972
Mw(kda)	98 kDa

Synonyms	JAK2, Janus kinase-2, Just Another Kinase 2
Uniprot	O60674

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Tono,C.,et al., Leukemia 19 (10), 1843-1844 (2005). 2. Goerttler,P.S., et al., Blood 106 (8), 2862-2864(2005).

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200248	40451	20 ug	$412.50		Add to Cart

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