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Home / Proteins & Peptide

ITK, GST-tag

SKU : BHP18200243

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BPS Bioscience

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Mfr.No.	40445
Description	Human ITK (GenBank Accession No. NM_005546), a.a. 352-end, with N-terminal GST-tag, MW= 57 kDa, expressed in a Baculovirus infected Sf9 cell expression system. Host cell line: Sf9 cells Specific activity: 13 pmol/min/ug
Immunogen Region	352-end
Tag	N-terminal GST-tag
Formulation	25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 1.37 mM KCl, 0.05% Tween-20, 30% glycerol, 10 mM glutathione, and 3 mM DTT.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 mM HEPES (pH 7.5), 10 mM MgCl₂, 1 mM EGTA, 0.01% Brij-35, 200 uM ATP, 2 uM Tyr1 substrate (Z-lyte kinase assay kit from Invitrogen), 1 hour at room temperature. Avoid freeze/thaw cycles.
GenBank	NM_005546
Mw(kda)	57 kDa

Synonyms	ITK, IL2-inducible T-cell kinase , Lyk, TSK
Uniprot	Q08881

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. August, A. et al. Int J Biochem Cell Biol. 2002 Oct,34(10):1184-9. 2.Finkelstein, L D. et al. Trends Cell Biol. 2004 Aug,14(8):443-51.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200243	40445	10 ug	$390.50		Add to Cart

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