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Home / Proteins & Peptide

IDO1, His-Avi-Tag, Biotin Labeled

SKU : BHP18200727

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Mfr.No.	79081
Description	Human IDO1, also known as Indoleamine 2,3-dioxygenase 1, GenBank Accession No. NM_002164, a.a. 2-403(end) with an N-terminal His-Avi-Tag, expressed in an E. coli cell expression system. MW = 48 kDa.
Immunogen Region	2-403
Tag	His-Avi-Tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween 20, 20% glycerol
Species	Human
Application	Useful for studying enzyme kinetics, substrate specificity, and screening inhibitors.
Notes	The assay was performed by UV absorption using a recombinant IDO1 or TDO2 and L-Tryptophan substrate. The UV absorption signal at 321 nm is correlated with the amount of N-formylkynurenine reaction product of reaction. All of the reactions were conducted at room temperature. The 100 ul reaction mixture in IDO or TDO Assay Buffer contains ~40 nM IDO1 (200 ng/well for 71182 or 210 ng/well for 79081) or varying concentrations (0-320 nM) of IDO1, 900 tryptophan, and the coupled reaction components. The reaction mixture incubated for 165 min respectively while reading the UV absorption signal. For the negative control (blank), assay buffer was added instead of the enzyme. Absorption signals were measured using a Tecan Infinite M1000 plate reader. The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Storing diluted protein is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank	NM_002164
Mw(kda)	48 kDa

Synonyms	Indoleamine 2,3-dioxygenase 1, IDO1, IDO2, Indoleamine 2,3-Dioxygenase 2, Interferon Gamma, IFNG
Uniprot	P14902

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Lob, S. et al., Cancer Immunol. Immunother. 2009; 58(1): 153-157.2. Liu, X. et al., Blood. 2010; 115(17): 3520-3530.3. Flick, H.E., et al., Int. Nat. J. of Tryptophan Res. 2013; 6: 35-45.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200727	79081	50 ug	$500.50		Add to Cart

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