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Home / Proteins & Peptide

IDH1 (R132H), FLAG-tag

SKU : BHP18201530

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Mfr.No.	71099-1
Background	Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). IDH1 homodimer is an NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, as well as in peroxisomal reactions that consume 2-oxoglutarate. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
Immunogen Region	1-414(end)
Tag	C-terminal FLAG-tag
Description	Human Isocitrate Dehydrogenase (IDH1), also known as oxalosuccinate decarboxylase, GenBank Accession No. NM_005896, full length (a.a. 1-414(end)) with Arg to His mutation on a.a. 132 and C-terminal FLAG-tag, MW= 48 kDa, expressed in Sf9 cells via a baculovirus expression system. Specific activity: >= 53 pmol/min/ug
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 80 ug/ml FLAG peptide, 0.04% Tween-20 and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	IDH reductive activity was measured in 200 ul reaction containing 25 mM Tris (pH 7.4), 150 mM NaCl, 10 mM MgCl₂, 0.03% BSA, 1 mM alpha-Ketoglutarate, 10 M NADPH and IDH. Depletion of NADPH was monitored continuously at Abs340 nm for 20 min. Molar extinction coefficient of NADPH is 6,200 M^-1cm^-1. Avoid freeze/thaw cycles.
GenBank	NM_005896
Mw(kda)	48 kDa

Synonyms	Isocitrate Dehydrogenase 1, IDH1, oxalosuccinate decarboxylase, IDP
Uniprot	O75874

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Dang, L., et al. (2010). Trends Mol. Med. 16(9):387-97. 2. Paschka, P., et al. (2010). J. Clin. Oncol. 28(22):3636-43.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201530	71099-1	50 ug	$330		Add to Cart

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