Human Recombinant HSP60 Protein

Mfr.No.	SPR-104, SPR-104A, SPR-104B
Description	Active Human Recombinant HSP60 Protein
Alternative Names	60kDa chaperonin Protein, cb863 Protein, CPN60 Protein, GROEL Protein, GroEL Homolog Protein, HLD4 Protein, HSP65 Protein, HSPD1 Protein, HuCHA60 Protein, SPG13 Protein
Biological Activity	ATPase active
Cellular Localization	Mitochondrion Matrix
Certificate Of Analysis	This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.6uM phosphate liberated/hr/ug protein in a 200ul reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay.
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
Regulatory	RUO
Scientific Background	In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. HSP60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian HSP60 (1-3). Whereas mammalian HSP60 is localized within the mitochondria, plant HSP60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that HSP60 is present in so many different species. The common characteristics of the HSP60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures (4). These similarities are supported by recent studies where the single-ring human mitochondrial homolog, HSP60 with its co-chaperonin, HSP10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of HSP60-HSP10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES (5). Another important function of HSP60 and HSP10 is their protective functions against infection and cellular stress. HSP60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes (6-9).
Species	Human
Specificity	~60 kDa
Accession Number	BC003030
Concentration	Lot/batch specific. See included datasheet.
Purification	Affinity Purified
Expression System	E. coli
Bioactivity	ATPase active
Biological Activity Abreviation	Active
Biological Activity Comment	ATPase active
Cite This Product	Human Recombinant HSP60 Protein (StressMarq Biosciences, Canada, Cat # SPR-104A)
Mol Weight	~60 kDa
Nature	Recombinant

Gross Weight (kg)	1.4
Launch Date	2007/09/01
Net Weight (g)	0.05
Number Of Pubmed Citations	3
Swissprot	P10809
Target Details	HSP60
Gene Id	3329
Research Area	Cancer, Heat Shock, Cell Signaling, Chaperone Proteins, Tags and Cell Markers, Organelle Markers, Protein Trafficking
Antigen	HSP60
Target	HSP60

Adr Code	Non-hazardous
Country Of Origin	Canada
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Published Application	Functional Assay ; Western Blot Control ; ELISA
Published Species Reactivity	Human ; Human
Pubmed Id	23775284 ; 24066722 ; 21506099
Shipping	Blue Ice or 4ºC
Stock Product	Y
Tarriff Code	3822.19.0030
Un Code For Transport	Non-hazardous
Unspsc Code	12352202
Shipping Temperature	Blue Ice or 4°C
Buffer	20mM Phosphate Buffer, 150mM NaCl, 10% glycerol
Description	Accession Number: BC003030 Specificity: ~60 kDa Certificate of Analysis: This product has been certified >90% pure using SDS-PAGE analysis. The protein has ATPase activity at the time of manufacture of 3.6µM phosphate liberated/hr/µg protein in a 200µl reaction at 37°C (pH7.5) in the presence of 20ul of 1mM ATP using a Malachite Green assay. Country of Origin: Canada Gross Weight (kg): 1.4 Net Weight (g): 0.05 Stock Product: Y
Purity	>90%
Storage Buffer	20mM Phosphate Buffer, 150mM NaCl, 10% glycerol
Storage Temperature	-20°C

Immunogen Species	Homo Sapiens (Human)
Applications	ATPase Activity assay, Functional assay, WB, ELISA, SDS-PAGE

Reference	1. Hartl F.U. (1996) Nature. 381: 571-579. 2. Bukau B. and Horwich A.L. (1998) Cell. 92: 351-366. 3.Hartl F.U. and Hayer-Hartl M. (2002) Science. 295: 1852-1858. 4. Jindal S., et al. (1989) Molecular and Cellular Biol. 9: 2279-2283. 5. La Verda D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh H., et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7.Gupta S. and Knowlton A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris C.C. et al. (2006) Cell Stress Chaperones. 11: 116-128. 9. Lai H.C., et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297.

Product Manual	Datasheet
SDS

Have you used this product?

Rate it now..!!

Combination SKU	Supplier No.	Size	Your price	Quantity
BHP11900010-1	SPR-104A	50 ug	$194		Add to Cart
BHP11900010-2	SPR-104B	100 ug	$377		Add to Cart

Human Recombinant HSP60 Protein

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Recombinant HSP60 Protein

Rate it now..!!