Human Recombinant Alpha Synuclein PFFs (Type 2)

Mfr.No.	SPR-317, SPR-317B, SPR-317C, SPR-317E
Description	Active Human Recombinant Alpha Synuclein Pre-formed Fibrils (Type 2), Active Human Recombinant Alpha Synuclein Pre-formed Fibrils (Type 2)
Alternative Names	Alpha synuclein PFFs, Alpha synuclein PFF, Alpha synuclein aggregates, Alpha synuclein protein aggregates, Alpha synuclein aggregates, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, SYN protein, Parkinson disease familial 1 Protein
Biological Activity	Does not induce Lewy body inclusion formation in Sprague-Dawley rat primary hippocampal neurons. Thioflavin T emission curve shows only a small increase in fluorescence (indicative of alpha synuclein aggregation) when Type 2 alpha synuclein PFFs (SPR-317) are combined with alpha synuclein monomers (SPR-321 or SPR-316). Certain biological activities in other neuronal cells cannot be ruled out. Researchers should test compatibility prior to use.
Cellular Localization	Cytoplasm, Membrane, Nucleus
Certificate Of Analysis	Certified 95% pure using SDS-PAGE analysis.
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
Regulatory	RUO
Scientific Background	Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6).
Species	Human
Specificity	~14.46 kDa
Accession Number	NP_000336.1
Concentration	Lot/batch specific. See included datasheet.
Purification	Ion-exchange Purified
Expression System	E. coli
Amino Acid Sequence	MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVATVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Bioactivity	Does not induce Lewy body inclusion formation in Sprague-Dawley rat primary hippocampal neurons. Thioflavin T emission curve shows only a small increase in fluorescence (indicative of alpha synuclein aggregation) when Type 2 alpha synuclein PFFs (SPR-317) are combined with alpha synuclein monomers (SPR-321 or SPR-316). Certain biological activities in other neuronal cells cannot be ruled out. Researchers should test compatibility prior to use.
Biological Activity Abreviation	Active
Biological Activity Comment	Does not induce Lewy body inclusion formation in Sprague-Dawley rat primary hippocampal neurons. Thioflavin T emission curve does not show an increase in fluorescence (which would indicate alpha synuclein aggregation) when control alpha synuclein PFFs (SPR-317) are combined with active alpha synuclein monomer (SPR-321) or control alpha synuclein monomer (SPR-316). Certain biological activities in other neuronal cells cannot be ruled out. Researchers should test compatibility prior to use.
Cite This Product	Human Recombinant Alpha Synuclein Protein (StressMarq Biosciences, Canada, Cat # SPR-317B)
Mol Weight	~14.46 kDa
Nature	Recombinant
Other Relevant Information	For best results, sonicate immediately prior to use. Refer to the Neurodegenerative Protein Handling Instructions on our website, or on the product datasheet for further information..
Protein Length	Full Length

Gross Weight (kg)	1.4
Launch Date	2016/11/08
Net Weight (g)	0.1
Other Relevant Information	Endotoxin below 1EU/µg as determined by LAL assay.
Swissprot	P37840
Target Details	Alpha Synuclein
Gene Id	6622
Research Area	Tangles & Tau, Multiple System Atrophy, Alzheimer's Disease, Neurodegeneration, Parkinson's Disease, Synuclein, Neuroscience
Antigen	Alpha Synuclein
Target	Alpha Synuclein

Adr Code	Non-hazardous
Country Of Origin	Canada
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Shipping	Blue Ice or 4ºC
Stock Product	Y
Tarriff Code	3822.19.0030
Un Code For Transport	Non-hazardous
Unspsc Code	12352202
Shipping Temperature	Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
Buffer	PBS pH 7.4
Description	Accession Number: NP_000336.1 Specificity: ~14.46 kDa Certificate of Analysis: Certified 92% pure using SDS-PAGE analysis. Country of Origin: Canada Gross Weight (kg): 1.4 Net Weight (g): 0.1 Stock Product: Y Other relevant information: Endotoxin below 1EU/µg as determined by LAL assay.
Purity	>95%
Storage Buffer	PBS pH 7.4
Storage Temperature	-80°C

Immunogen Species	Homo Sapiens (Human)
Applications	In vivo assay, In vitro assay, WB, SDS-PAGE

Reference	1. Genetics Home Reference: SNCA. US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757.

Product Manual	Datasheet
SDS

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Combination SKU	Supplier No.	Size	Your price
BHP11900127-1	SPR-317B	100 ug	$390	Add to Cart
BHP11900127-2	SPR-317C	100 ug x2	$700	Add to Cart
BHP11900127-3	SPR-317E	100 ug x5	$1455	Add to Cart

Human Recombinant Alpha Synuclein PFFs (Type 2)

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