Alpha Synuclein Protein

Mfr.No.	SPR-326, SPR-326B, SPR-326C, SPR-326E
Description	Active Human Recombinant A53T Mutant Alpha Synuclein Protein Pre-formed Fibrils (Type 1)
Alternative Names	A53T mutant alpha synuclein, A53T mutated SNCA, A53T Alpha synuclein PFFs, Alpha synuclein PFF, Ala53thr mutant alpha synuclein, Alpha synuclein pre-formed fibrils, Alpha synuclein aggregates, Alpha synuclein protein aggregates, Alpha synuclein aggregates, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, SYN protein, Parkinson disease familial 1 Protein
Biological Activity	100 uM A53T alpha synuclein protein monomer (SPR-325) seeded with 10 uM A53T alpha synuclein protein PFF (SPR-326) in 25 uM Thioflavin T (PBS pH 7.4, 100 ul reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Cellular Localization	Cytoplasm, Membrane, Nucleus
Certificate Of Analysis	Certified >95% pure using SDS-PAGE analysis.
Field Of Use	Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
Scientific Background	Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial leaing and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). The A53T mutation is a missense point mutation where alanine is replaced by threonine at the 53rd amino acid. This mutation has been linked to early-onset Parkinson's Disease (7) and increased rates of alpha synuclein fibrillization (8).
Species	Human
Specificity	~14.46 kDa
Accession Number	NP_000336.1
Concentration	Lot/batch specific. See included datasheet.
Purification	Ion-exchange Purified
Expression System	E. coli
Amino Acid Sequence	MDVFMKGLSK AKEGVVAAAE KTKQGVAEAA GKTKEGVLYV GSKTKEGVVH GVTTVAEKTK EQVTNVGGAV VTGVTAVAQK TVEGAGSIAA ATGFVKKDQL GKNEEGAPQE GILEDMPVDP DNEAYEMPSE EGYQDYEPEA
Bioactivity	100 uM A53T alpha synuclein protein monomer (SPR-325) seeded with 10 uM A53T alpha synuclein protein PFF (SPR-326) in 25 uM Thioflavin T (PBS pH 7.4, 100 ul reaction volume) generated a fluorescence intensity of 28 000 Relative Fluorescence Units after incubation at 37°C with shaking at 600 rpm for 56 hours. Fluorescence was measured by excitation at 450 nm and emission at 485 nm on a Molecular Devices Gemini XPS microplate reader.
Biological Activity Abreviation	Active
Cite This Product	Human Recombinant A53T Alpha Synuclein Protein PFFs (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-326B)
Mol Weight	~14.46 kDa
Nature	Recombinant
Other Relevant Information	For best results, sonicate immediately prior to use. Refer to the Neurodegenerative Protein Handling Instructions on our website, or on the product datasheet for further information..
Protein Length	Full Length

Gross Weight (kg)	1.4
Launch Date	2019/02/20
Net Weight (g)	0.1
Swissprot	P37840
Target Details	Alpha Synuclein A53T Mutant
Gene Id	6622
Research Area	Tangles & Tau, Multiple System Atrophy, Alzheimer's Disease, Neurodegeneration, Parkinson's Disease, Synuclein, Neuroscience
Target	Alpha Synuclein

Adr Code	Non-hazardous
Country Of Origin	Canada
Stock Product	Y
Tarriff Code	3822.19.0030
Un Code For Transport	Non-hazardous
Unspsc Code	12352202
Shipping Temperature	Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
Purity	>95%
Storage Buffer	PBS pH 7.4
Storage Temperature	-80°C

Immunogen Species	Homo Sapiens (Human)
Applications	In vivo assay, In vitro assay, WB, SDS-PAGE

Reference	1. Genetics Home Reference: SNCA. US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. 7. Polymeropoulos, M. H. (1998). Science. 276(5321), 20452047 8. Conway, K.E., et al. (1998). Nat Med. 4(11):1318-20

Product Manual	Datasheet

No Description Available.

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Combination SKU	Supplier No.	Size	Your price
BHP11900164-1	SPR-326B	100 ug	$382	Add to Cart
BHP11900164-2	SPR-326C	100 ug x2	$611	Add to Cart
BHP11900164-3	SPR-326E	100 ug x5	$1240	Add to Cart

Human Recombinant A53T Mutant Alpha Synuclein Protein Pre-formed Fibrils (Type 1)

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