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Home / Proteins & Peptide

Human IL-12 (IL-12A & IL-12B) protein, His Tag

SKU : BHP13700102

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Abbkine Scientific Co., Ltd.

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Mfr.No.	PRP100705
Description	Human IL-12 (IL-12A & IL-12B) protein, His Tag, expressed in HEK293 Cells
Alternative Names	CLMF; CLMF2; IL-12A; IL-12B; IL12; IMD28; IMD29; NFSK; NKSF; NKSF1; NKSF2; P35; Interleukin-12 subunit alpha & Interleukin-12 subunit beta; IL12A & IL12B
Background	IL-12 is composed of a bundle of four alpha helices. It is a heterodimeric cytokine encoded by two separate genes, IL-12A (p35) and IL-12B (p40). The active heterodimer (referred to as 'p70'), and a homodimer of p40 are formed following protein synthesis.IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) from T cells and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-gamma. T cells that produce IL-12 have a coreceptor, CD30, which is associated with IL-12 activity. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 mediates enhancement of the cytotoxic activity of NK cells and CD8+ cytotoxic T lymphocytes. There also seems to be a link between IL-2 and the signal transduction of IL-12 in NK cells. IL-2 stimulates the expression of two IL-12 receptors, IL-12R-beta1 and IL-12R-beta2, maintaining the expression of a critical protein involved in IL-12 signaling in NK cells. Enhanced functional response is demonstrated by IFN-gamma production and killing of target cells.
Immunogen Region	PRP100705
Tag	IL12A & IL12B
Formulation	Lyophilized from sterile PBS, pH 7.4
Molecular Weight	60.2 (24 + 36.2) kDa
Usage	Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL- 12 (IL12A & IL12B Heterodimer) protein in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Accession Number	NP_000873.2/NP_002178.2
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Bioactivity	Measured by its ability to bind biotinylated recombinant human IL-12RB1 in a functional ELISA. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3 stimulated PBMC.The ED50 for this effect is typically 0.1-0.6 ng/mL.2. Measured in a cell proliferation assay using Anti-CD28 and Anti-CD3 stimulated PBMC.The ED50 for this effect is typically 0.1-0.6 ng/mL.
Protein Length	The recombinant IL-12 heterodimer of human IL-12A/IL-12B comprises 525 (208 + 317) amino acids and has a calculated molecular mass of 60.2 (24 + 36.2) kDa. The apparent molecular mass of rh IL-12 heterodimer is approximately 40 & 43 kDa respectively in SDS-PAGE under reducing conditions.
Others	The Endotoxin level is less than 1.0 EU per ug of the protein as determined by the LAL method Preparation Method: HEK293 Cells

Sequence	Amino acid sequence derived from p35 subunit of human IL-12, termed as IL-12A (NP_000873.2) (Met 1-Ser 219) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1 ; Amino acid sequence derived from p40 subunit of human IL-12, termed as IL-12B (NP_002178.2) (Met 1-Ser 328) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Purity	> 95 % as determined by SDS-PAGE
Storage	Lyophilized Human IL-12 (IL-12A & IL-12B) protein, His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.96% HSA or BSA). Please prevent freeze-thaw cycles.

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SKU	Supplier No.	Size	Your price	Quantity
BHP13700102	PRP100705	50 ug	$1029		Add to Cart
BHP13700102	PRP100705	500 ug	$8749		Add to Cart

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