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Home / Proteins & Peptide

Human IL-10 protein, His Tag

SKU : BHP13700068

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Abbkine Scientific Co., Ltd.

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Mfr.No.	PRP100227
Description	Human IL-10 protein, His Tag, expressed in HEK293 Cells
Alternative Names	CSIF; GVHDS; IL-10; IL10A; Interleukin-10; TGIF; Interleukin-10; IL10
Background	IL-10 is a anti-inflammatory cytokine which belongs to the IL-10 family. It is produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types, while it is produced primarily by monocytes and to a lesser extent by lymphocytes. IL-10 is mainly expressed in monocytes and Type 2 T helper cells (TH2), mast cells, CD4+CD25+Foxp3+ regulatory T cells, and also in a certain subset of activated T cells and B cells. IL-10 has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract. The importance of interleukin 10 for counteracting excessive immunity in the human body is revealed by the fact that patients with Crohn's disease react favorably towards treatment with bacteria producing recombinant IL-10. IL-10 inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. It also displays a potent ability to suppress the antigen-presentation capacity of antigen presenting cells. However, it is also stimulatory towards certain T cells and mast cells and stimulates B cell maturation and antibody production.
Immunogen Region	PRP100227
Tag	IL-10
Formulation	Lyophilized from sterile PBS, pH 7.4
Molecular Weight	20.9 kDa
Usage	Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL10 protein, His Tag in sterile ddH2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Accession Number	NP_000563.1
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Bioactivity	1. Immobilized human IL-10-His at 10 ug/ml (100 ul/well) can bind Cynomolgus IL-10RA-Fc, The EC50 of Cynomolgus IL-10RA-Fc is 0.31-0.71 ug/ml.2. Measured in a cell proliferation assay using MC/9-2 mouse mast cells. The ED50 for this effect is typically 5-20 ng/mL.
Protein Length	The recombinant human IL-10 comprises 176 amino acids and has a predicted molecular mass of 20.9 kDa as estimated in SDS-PAGE under reducing conditions.
Others	The Endotoxin level is less than 1.0 EU per ug of the protein as determined by the LAL method Preparation Method: HEK293 Cells

Sequence	Amino acid sequence derived from human IL-10 (NP_000563.1) (Ser 19-Asn 178) was expressed, with a N-terminal polyhistidine tag.

Purity	> 85 % as determined by SDS-PAGE
Storage	Lyophilized Human IL-10 protein, His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.59% HSA or BSA). Please prevent freeze-thaw cycles.

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SKU	Supplier No.	Size	Your price	Quantity
BHP13700068	PRP100227	100 ug	$1819		Add to Cart
BHP13700068	PRP100227	1 mg	$8569		Add to Cart

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