In cooperation with other chaperones, Hsp70 stabilizes preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
Immunogen Region
2-641
Tag
C-terminal His-tag
Description
Human Heat Shock Protein 70 (GenBank Accession No. NM_005345), a.a. 2-641 with C-terminal His-tag, MW = 71 kDa, expressed in E. coli.
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Avoid freeze/thaw cycles.
GenBank
NM_005345
Mw(kda)
71 kDa
Synonyms
HSP70, Heat Shock Protein 70, DnaK, HSP70i
Uniprot
P08107
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Lee K.J. et al., Exp. Mol. Med. 40 (6), 658-668 (2008). 2. Birtas-Atesoglu E. et al., Clin. Exp. Rheumatol. 26, S96-S98 (2008).