Human recombinant histone H3 (a.a. 1-136(end)) with K27C, C97A, and C111A mutations, and an N-terminal His-tag, expressed in an E. coli expression system. GenBank Accession No. NM_003532. The protein has been alkylated to produce the lysine analog, dimethyl aminoethylcysteine. MW = 16 kDa.
Host cell line: E. coli
Formulation
PBS (8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl), 20% glycerol.
Mutation
K27C
Species
Human
Application
A perfect substrate for histone methyltransferases and demethylases.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_003532
Mw(kda)
16 kDa
Synonyms
Histone 3.1, HIST1H3E
Uniprot
P68431
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Simon, M.D., et al. Cell 2007; 128: 1003-1012. 2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46