Human recombinant histone tetramer consisting of 2 molecules each of histones H3 (a.a. 2-136(end)) and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, aminoethylcysteine. GenBank Accession Nos. NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Global MW = 57 kDa.
Host cell line: E. coli
Tag
N-terminal His-tag
Formulation
45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol
Species
Human
Application
A perfect substrate for histone methyltransferases and demethylases.
Notes
Avoid freeze/thaw cycles.
GenBank
NM_033445, NM_003528, NM_003532, and NM_003548
Mw(kda)
57 kDa
Synonyms
H3/H4
Uniprot
P68431
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Simon, M.D., et al. Cell 2007; 128: 1003-1012. 2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46