Human HDAC9 , GenBank Accession No. NM_178423, a.a. 604-1066 with C-terminal His-tag, MW= 50.7 kDa, expressed in baculovirus expression system.
Host cell line: Sf9 cells
Specific activity: >=3000 pmol/min/ug
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 200 mM imidazole
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 uM BPS HDAC class 2a substrate (Catalog #50040), and HDAC9 (0.15 - 10 ng). Incubation condition: 30 min at 37°C, followed byHDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.