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Home / Proteins & Peptide

GM-CSF, Human

SKU : BHP18500002

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Mfr.No.	IX00349, IX00349-1
Description	Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils.Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can induce human endothelial cells to migrate and proliferate. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines.
Reconstitution	It is recommended that the lyophilized Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) be reconstituted in sterile 18 Momega-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Formulation	The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.0, 150mM NaCl, 5% Mannitol buffer.
Molecular Weight	14,000 Da
Source	E. coli
Host	Human
Endotoxin Level	Less than 0.03 ng/ug (0.3 IEU/ug) determined by LAL test
Bioactivity	The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.1 ng/ml, corresponding to a Specific Activity of 1.0x107 IU/mg.

Synonyms	rHuGM-CSF; GM-CSF
Sequence	MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNETVEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTMMASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPFDCWEPVQE

Purity	Greater than 98% as determined by(a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel
Storage	Lyophilized Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF), although stable at room temperature for three weeks, should be stored desiccated at -18°C or below. Upon reconstitution, Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) may be stored at 4°C for short periods (two to seven days). For long term storage, it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP18500002-1	IX00349-1	1mg	$2621		Add to Cart

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