Dephosphorylated recombinant FGFR3 (Fibroblast Growth Factor Receptor 3) encompassing amino acids 397-806. This protein contains the pathogenic mutation N540K in the tyrosine kinase domain. It also contains an N-terminal His-Tag. This protein was co-expressed with PTP1B for dephosphorylation. Phosphorylation level confirmed to be<5%. The protein is biotinylated using an N-terminal Avi-Tag. Constructs: FGFR3 (N540K) (His-397-806(end)) / PTP1B (GST-Th-1-435(end))
Background
The N540K mutation results in increased FGFR3 autophosphorylation and substrate phosphorylation, and has been linked to hypochondroplasia (short-limbed dwarfism) and impaired chondrocyte differentiation.
Immunogen Region
397-806(end)
Tag
N-terminal His-tag
Formulation
40 mM Tris-HCl pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 3 mM DTT