Human F-Box Protein 11 (FBXL11), alsoknown as KDM2A, Ndy2, and JHDM1AGenBank Accession # NM_012308, a.a. 2-700 with N-terminal FLAGAvi-tag, MW = 82 kDa, expressed in Sf9cells via a baculovirus expressionsystem.
Host cell line: Sf9 cells
Specific activity: 0.01 pmole/min/ug
Immunogen Region
2-700
Tag
N-terminal FLAG-Avi-tags
Formulation
40 mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2 mM KCl, 0.04% Tween-20,20% glycerol, 80 ug/ml FLAG peptide
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
The assay wasperformed using BiohippoFBXL11 Homogeneous Assay Kit (Cat#50611) and following themanufacturer's recommended protocol.
Avoidfreeze/thaw cycles. Storing diluted enzyme isnot recommended, if necessary, use carrierprotein (BSA 0.1 - 0.5%).
GenBank
NM_012308
Mw(kda)
82 kDa
Synonyms
F-Box Protein 11, KDM2A, Ndy2, JHDM1A
Uniprot
Q9Y2K7
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Tanaka, Y., et al. (2010). EMBO J. 29(9):1510-22. 2. Blackledge, N.P., et al. (2010). Mol. Cell. 38(2):179-90. 3. Frescas, D., et al. (2008). Cell Cycle 7(22):3539-47.