Human F-Box Protein 10 (FBXL10, also known as KDM2B, JHDM1B, CXXC2, and Histone-H3 K36 demethylase 1B), GenBank Accession No. NM_032590, a.a. 1-650, with C-terminal FLAG-tag, MW= 75 kDa, expressed in Sf9 cells via a baculovirus expression system.
Host cell line: Sf9 cells
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ug/ml FLAG peptide
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Enzyme wasincubated with 0.4 uM substrate inHEPES-containing JMJ assay buffer SAand SA- (without KG) in a 10-ul reactionfor 180 min at 30°C. 5 ul of Anti-RabbitAlphaLISA acceptor beads (1:250) and 5ul of rabbit polyclonal antibody (1:200)were added and incubation continued for30 min. Streptavidin Donor beads (1:125)were added (10 ul) and mixture was furtherincubated for 10 min at RT before Acountswere measured.