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Home / Proteins & Peptide

EZH1/EED/SUZ12/RbAp48/AEBP2, His-tag, FLAG-tag

SKU : BHP18200407

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Mfr.No.	51007
Description	Complex of human EZH1, GenBank Accession No. NM_001991, a.a. 2-end, with N-terminal His-tag, MW= 86 kDa, human EED, NM_003797, a.a. 2-end, with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12, NM_015355, a.a. 2- end, with N-terminal His-tag, MW = 87 kDa, human AEBP2, NM_153207, a.a. 2-end, with N-terminal His-tag, MW= 53 kDa, and human RbAp48, NM_005610, a.a. 2-end, with N-terminal His-tag, MW = 48 kDa, co-expressed in baculovirus expression system. Host cell line: Sf9 cells
Immunogen Region	2-end (all components)
Tag	N-terminal His-tag (EZH1, SUZ12, AEBP2, RbAp48), N-terminal FLAG-tag (EED)
Formulation	40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 200 mM imidazole,and 20% glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (20 mM phosphate pH 7.4, 0.05% Tween-20, 40 uM S-adenosylmethionine, and enzyme complex) add to the wells coated with the substrate on a Neutravidin white plate (G-Biosciences). Incubate for 1hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. Avoid freeze/thaw cycles.
GenBank	NM_001991 (EZH1), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
Mw(kda)	325 kDa (complex)

Synonyms	KMT6B, EZH, Retinoblastoma Binding Protein 4 (RBBP4)
Uniprot	Q92800

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Margueron R. et al. Mol. Cell 32, 4, 503-518, 2008. 2. Shen X. et al., Mol. Cell 32, 4, 491-502, 2008.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200407	51007	50 ug	$1078		Add to Cart

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