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Home / Proteins & Peptide

ExKine Membrane and Cytoplasmic Protein Extraction kit

SKU : BHE13700061

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTP3005
Features	Versatilesuitable for fresh mammalian cultured cells and tissues with little or no cross-contaminations. Fast and convenientnon-denatured, active proteins are purified in less than two hours. Compatibleapply in various downstream assays, including Western blotting, protein assays, enzyme activity assays, etc.
Background	The preparation of an extract from membrane is often the first step in studying membrane proteins and their interactions. The resulting preparation can be used directly in Western blotting, Dot blot, Immunoprecipitation, or as a starting point for the purification of specific proteins. The preparation of an extract from Cytoplasm is often the first step in studying Cytoplasmic proteins and their interactions. The resulting preparation can be used directly in Western blotting, Electrophoresis Mobility Shift Assay (EMSA), footprinting analysis, transcription assays, or as a starting point for the purification of regulatory proteins.
Tag	Cytoplasmic
Description	Abbkine ExKine Membrane and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of cytoplasmic and membrane protein.
Usage	Perform all steps at 28 °C. Use precooled buffers and equipment. Ensure all the solutions are defrosted and homogeneous.
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Notes	Abbkine ExKine Membrane and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of non-denatured, active cytoplasmic and membrane protein from mammalian cultured cells or tissues, which can be used directly in a variety of proteomics application. The cells are first broken by homogenization to allow the release of cytosolic and membrane proteins, after which membrane proteins are obtained by high speed centrifugation. Extraction efficiencies and yields will vary depending on cell type as well as the number of times the integral membrane protein spans the lipid bilayer.
Kit Components	Cytoplasmic Extraction Buffer (CEB) (5x) Membrane Extraction Buffer (MEB) Protease Inhibitor (100x)

Storage	Refer to list of materials supplied for storage conditions of individual components. Stable for at least 12 months at recommended temperature from date of shipment. Gel pack with blue ice.

Description	Abbkine ExKine™ Membrane and Cytoplasmic Protein Extraction Kit enable stepwise separation and preparation of non-denatured, active cytoplasmic and membrane protein from mammalian cultured cells or tissues, which can be used directly in a variety of proteomics application. The cells are first broken by homogenization to allow the release of cytosolic and membrane proteins, after which membrane proteins are obtained by high speed centrifugation. Extraction efficiencies and yields will vary depending on cell type as well as the number of times the integral membrane protein spans the lipid bilayer.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13700061	KTP3005	50 T	$119		Add to Cart
BHE13700061	KTP3005	200 T	$279		Add to Cart

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