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Home / Proteins & Peptide

EHMT1 (GLP), GST-tag

SKU : BHP18200418

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BPS Bioscience

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Mfr.No.	51020
Description	Human euchromatic histone-lysine N-methyltransferase (EHMT1), also known as G9a-like protein, GLP or KMT1D, GenBank Accession No. NM_024757, a.a. 894-1298 (end) with N-terminal GST-tag, MW= 72.4 kDa, expressed in an E. coli expression system. Host cell line: E. coli Specific activity: >=122.3 pmole/min/ug
Immunogen Region	894-1298 (end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 16 mM glutathione, 20% glycerol, and 3 mM DTT
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	50 ul reaction mix (50 mM Tris pH 8.8, 5 mM MgCl₂, 4 mM DTT, 0.05% Tween-20, 40 uM S-adenosylmethionine, and EHMT1 (0.05 - 0.5 ng)) is added to substrate-coated wells on a Neutravidin black plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K9 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence. Avoid freeze/thaw cycles.
GenBank	NM_024757
Mw(kda)	72.4 kDa

Synonyms	EHMT1, GLP, KMT1D, G9a-like protein, euchromatic histone-lysine N-methyltransferase
Uniprot	Q9H9B1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Huang, J., et al., J Biol. Chem. 2010 Mar 26; 285(13): 9636-41. 2. Ueda, J., et al., J Biol. Chem. 2006 Jul 21; 281(29): 20120-8.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200418	51020	50 ug	$484		Add to Cart

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