Human DRAK2, also known as DAP kinase-related apoptosis-inducing protein kinase 2 or death-associated protein kinase-related 2 (GenBank Accession No. NM_004226.3), a.a. 2-372 (end), MW = 43 kDa, expressed in a baculovirus-infected Sf9 cell expression system.
Immunogen Region
2-372 (end)
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, 1 mM DTT, 1 mM EDTA
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Assay was done in Kinase buffer containing 1 mM DTT using MRCL3 peptide substrate (0.2 mg/ml) and 20uM ATP. Reaction was done at 30°C for 50 min. Amount of ATP transferred was calculated using Kinase-Glo reagent (Promega)
Avoid freeze/thaw cycles.
GenBank
NM_004226.3
Mw(kda)
43 kDa
Synonyms
STK17B, DAP kinase-related apoptosis-inducing protein kinase 2 or death-associated protein kinase-related 2
Uniprot
O94768
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1-0.5%).
Reference
1. McGargill, M. et al, Immunity. 21 (6): 781-791 (2004).2. Mao, J. et al, Journal of Biological Chemistry. 281: 12587-12595 (2006).