Recombinant human DPP9 (dipeptidyl peptidase 9), isoform 1 (short form), encompassing amino acids 1-863(end). This construct contains an N-terminal GST-tag. The recombinant protein was affinity purified, expressed in a Baculovirus infected Sf9 cell expression system.
Specific activity: >= 536 pmol/min/ug
Background
Dipeptidyl peptidase-9 (DPP9) is a serine exopeptidase that cleaves X-prolinedipeptides from the N-terminus of polypeptides. DPP9 exhibits similar activity tothat of DPP4 but unlike DPP4, DPP9 is not membrane bound. DPP9 activity has beenlinked to several diseases, including type 2 diabetes, obesity and cancer.
Immunogen Region
full length
Tag
N-terminal GST-tag
Formulation
45 mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4 mM KCl, 18 mMglutathione, 10% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
25 mM Tris HCl,pH 7.5, 0.1% BSA, 5 uM substrate (H-Gly-Pro-AMC), and DPP9. Incubationcondition: 1 min. at room temperature.
Avoid freeze/thaw cycles.
GenBank
NM_001384623
Mw(kda)
124 kDa
Synonyms
Dipeptidylpeptidase 9, DPPIX
Uniprot
Q86TI2-1
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Bjelke,J.R. et al., Biochem. J. 396 (2), 391-399 (2006). 2. Yu, D.M. et al., FEBS J. 273 (11), 2447-2460(2006).