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Home / Proteins & Peptide

Dog PDE4B, GST-tag

SKU : BHP18200517

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BPS Bioscience

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Mfr.No.	60055
Description	Dog phosphodiesterase 4B (PDE4B) GenBank Accession No. XP_003434692, a.a. 2-737(end), with N-terminal GST-tag, MW 110 kDa, expressed in Sf9 insect cells via a baculovirus expression system. host species: Sf9 cells Host species: Sf9 cells Specific activity: >= 3882 pmol/min/ug Unit definition: One unit is defined as the amount of enzyme that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.
Immunogen Region	2-737(end)
Tag	N-terminal GST-tag
Formulation	40 mM Tris pH 8, 110 mM NaCl, 2.2 mM KCl, 20% glycerol, 3 mM DTT
Species	Dog
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	10 mM Tris-HCl, pH 7.4, 10 mM MgCl₂, 0.1 mg/ml BSA, 0.05% Tween-20, 200 uM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of dPDE4B at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Avoid freeze/thaw cycles.
GenBank	XP_003434692
Mw(kda)	110 kDa

Synonyms	PDE4B, phosphodiesterase 4B
Uniprot	F1PJ12

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Girgenti, M.J., et al., PLoS One 2012,7(2):e32404. 2. Zhao, H., et al., Eur. J. Pharmacol. 2008 Aug 20,590(1-3):29-35.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200517	60055	5 ug	$517		Add to Cart

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