Human Dicer, also known as Dicer 1, Ribonuclease III, GenBank Accession No. NM_030621, a.a. 2-1992 (end), with a N-terminal His-Tag, expressed in an Sf9 cell expression system. MW =221 kDa.
host species: insect
Host species: insect
Host cell line: Sf9
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, 40 mM imidazole
Species
Human
Notes
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
NM_030621
Mw(kda)
221 kDa
Synonyms
Dicer 1, Ribonuclease III
Uniprot
Q9UPY3
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C. Avoid freeze/thaw cycles. Protein may be diluted to>= 100 ug/ml in PBS + glycerol and stored at -80°C.
Reference
1. Hutagnr, G., et al. Science. 2001; 293(5531): 834-838.2. Bernstein, E., et al., Nat. Genetics. 2003; 35: 215-217 (2003).