Complex of human cullin-3 (CUL3) with human RING box protein 1 (Rbx1), also known as E3 ubiquitin-protein ligase RBX1. CUL3 (a.a. 2-768(end) with N-terminal GSTtag, 117 kDa) is co-expressed with Rbx1 (a.a. 1-108(end), 12 kDa) in a baculovirus infected Sf9 cell expression system. GenBank Accession Nos. are NM_003590 (CUL3) and NM_014248 (Rbx1).
Immunogen Region
CUL3: 2-768(end); Rnx1: 1-108(end)
Tag
N-terminal GST-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and 1.6 mM glutathione
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended. If necessary, use carrier protein (BSA 0.1 - 0.5%).
GenBank
CUL3: NM_003590; Rbx1: NM_014248
Mw(kda)
CUL3: 117 kDa; Rbx1: 12 kDa
Synonyms
Cullin-3, RING box protein 1, RING box protein 1, E3 ubiquitin protein ligase 1
Uniprot
CUL3: Q13618; Rbx1: P62877
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Kaspar, J. et al., J. Biol. Chem. 2010; 285: 21349-213582. Kaspar, J. et al., J. Cell. Sci. 2012; 125: 1027-1038.