Human cMet (GenBank Accession No. X54559), a.a. 956-1390 with N-terminal GST-tag, MW= 74 kDa, expressed in an Baculovirus infected Sf9 cell expression system.
Host cell line: Sf9 cells
Specific activity: 64 pmol/min/ug
Formulation
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 10% glycerol and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
cMet was incubated with 2 uM substrate (Tyr2 peptide from Invitrogen) for 1 hour at 30%deg;C in a buffer containing 50 mM HEPES (pH 7.5), 10 mM MgCl2, 1 mM EDTA, 0.01% BRIJ-35 and 200 uM ATP. Developer solution was added to reaction and reaction was stopped after 1 hour of incubation at room temperature.