Recombinant human cereblon (CRBN), full length, encompassing amino acids 2-422(end). This construct contains an N-terminal Flag-tag. The recombinant protein was affinity purified.
host species: HEK293
Host species: HEK293
Immunogen Region
2-442(end)
Tag
N-terminal FLAG-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 20% glycerol, and 100 ug ml FLAG peptide
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
Notes
Assay was performed according to Cereblon Binding Assay Kit (Biohippo #79899) with CRBN titrated from 2400 ng/rxn - 0 ng/rxn incubated for 90 minutes at room temperature. Fluorescence polarization measured at ex635 nm, em682 nm.