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Home / Proteins & Peptide

Cereblon/DDB1/Cul4A/Rbx1 Complex

SKU : BHP18201133

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BPS Bioscience

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Mfr.No.	100329-1
Description	Cereblon/DDB1/Cul4A/Rbx1 complex of human Cereblon (GenBank Accession No. NM_016302) a.a. 2-442(end) with N-terminal FLAG-tag and MW= 51 kDa, human DDB1 (GenBank Accession No. NM_001923), a.a. 1-1140(end) with N-terminal FLAG-tag and MW= 128 kDa, human Cul4A (GenBank Accession No. NM_003589), a.a. 2-759(end) with N-terminal His-tag and MW= 87 kDa, human Rbx1 (GenBank Accession No. NM_014248), a.a. 2-108(end) with N-terminal His-tag and MW= 13 kDa, co-expressed in a HEK293 expression system.
Immunogen Region	Cereblon: 2-442(end); DDB1: 1-1140(end); Cul4a: 2-759(end); Rbx1: 2-108(end)
Tag	Cereblon: N-terminal FLAG-tag; DDB1: N-terminal FLAG-tag; Cul4a: N-terminal His-tag; Rbx1: N-terminal His-tag
Formulation	40 mm Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20 and 20% Glycerol.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	PROTAC ternary complex formation with BRD3 and cereblon was assessed using AlphaScreen technology using FLAG-acceptor beads and GSH-donor beads. Various amounts of Cereblon were incubated in 10-ul reaction with 100 nM dBET1 and 15 nM GST-BRD3 BPS #31033 at room temperature for one hour. After this, FLAG-acceptor beads and GSHdonor beads were added followed by detection of A-counts. Avoid freeze/thaw cycles.
GenBank	Cereblon: NM_016302; DDB1: NM_001923; Cul4a: NM_003589; Rbx1: NM_014248
Mw(kda)	Cereblon: 51 kDa; DDB1: 128 kDa; Cul4a: 87 kDa; Rbx1: 13 kDa

Synonyms	CRBN, Protein X 0001, MRT2A MRT2
Uniprot	Cereblon:Q96SW2; DDB1: Q16531; Cul4a: Q13619; Rbx1: P62877

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Gang, Lu., et al., Science. 2014; 343(6168): 305-309.2. Zhu, Y.X., et al., Blood. 2011; 118: 4771-4779.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201133	100329-1	10 ug	$280.50		Add to Cart

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