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Home / Proteins & Peptide

CD40, Fc Fusion (IgG1) Avi-Tag, Biotin Labeled

SKU : BHP18201705

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BPS Bioscience

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Mfr.No.	79102-2
Description	Human CD40, also known as Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, Bp50. GenBank Accession No. NM_001250, a.a. 21-193, fused at the C-terminus with the Fc portion of Human IgG1, with C-terminal Avi-tag, expressed in a HEK293 cell expression system. MW = 48 kDa. This protein runs at a higher molecular weight due to glycosylation.
Immunogen Region	21-193
Tag	Fc (IgG1), Avi-Tag, Biotin Labeled
Formulation	8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol.
Species	Human
Application	Useful for Western blots, studying protein binding, and for screening small molecules and antibodies.
Endotoxin Level	<1 EU/ug
Notes	Reaction done according to the protocol for the CD40:CD40L(Biotinylated) Inhibitor Screening Assay Kit, #79257). Plate was coated with 100 ng/well CD40L (#71191) at 4°C overnight. Various concentrations of biotinylated CD40 (0-1 uM) added for 2h at RT. Strep-HRP added for 1h at RT. The aggregation state of this product has been confirmed to be<10%. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank	NM_001250
Mw(kda)	48 kDa

Synonyms	CD40, Tumor Necrosis Factor Receptor Superfamily Member 5, TNFRSF5, Bp50,
Uniprot	P25942

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Lutgens, E. et al., Nat. Med. 1998; 5: 1313-1316.2. Quezada, S.A., et al., Immunology. 2003; 22: 307-328

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201705	79102-2	50 ug	$478.50		Add to Cart

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