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Home / Proteins & Peptide

Cathepsin F, His-tag

SKU : BHP18200777

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BPS Bioscience

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Mfr.No.	80003
Description	Human cathepsin F (GenBank Accession No. NM_003793), CD33 signal peptide(a.a. 1-16) + cathepsin F (a.a. 20-end), with C-terminal His-tag, MW = 53 kDa (more than 58kDa after post-modification), expressed in FreeStyle 293-F cells. Specific activity: >= 0.9 pmole/min/ug.
Immunogen Region	CD33 1-16, CathepsinF 20-end
Tag	C-terminal His-tag
Formulation	45 mM Na Acetate pH 5.5, 135 mM NaCl and 10% glycerol
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Enzyme activity wasmeasured in a buffer containing 100 mMSodium acetate (pH 5.5), 2 mM EDTA, 8 mMDTT, 0.05% Brij, and 20 uM substrate (Z-FRAMC),with 3.75-30 ng/ul Cathepsin F.Reaction was done at 30°C for 40-120min, and fluorescence signal was measuredat Ex360 nm/Em460 nm. Enzyme waspreactivated in 50 mM sodium acetate pH4.5, 5 mM DTT buffer for 90 min at RT prior toreaction. Avoid freeze/thaw cycles.
GenBank	NM_003793
Mw(kda)	53 kDa

Synonyms	CTSF, CATSF
Uniprot	Q9UBX1

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Kaakinen R. et al., Atherosclerosis 192 (2), 323-327 (2007). 2. Vazquez-Ortiz G. et al., BMC Cancer 5, 68 (2005).Application Reference(s):1. Orbe, J., et al. J Med Chem . 2015 Apr 9;58(7):2941-57. doi: 10.1021/jm501939z. Discovery and safety profiling of a potent preclinical candidate, (4-(4-(((3R)-3-(hydroxycarbamoyl)-8-azaspiro(4.5)decan-3-yl)sulfonyl)phenoxy)-N-methylbenzamide) (CM-352), for the prevention and treatment of hemorrhage (2015)

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200777	80003	50 ug	$412.50		Add to Cart

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