Human carbonic anhydrase I, also known as CA1, (GenBank Accession No. NM_001128829), full length (a.a. 1-261(end)) with C-terminal His-tag, MW = 29.7 kDa, expressed in an E. coli cell expression system.
Specific activity: 2,000 pmol/min/ug
Immunogen Region
1-261(end)
Tag
C-terminal His-tag
Formulation
40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween-20, 185 mM imidazole and 20% glycerol
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
15 mM Tris-HCl, pH7.4, 100 mM NaCl, 3 mM p-Nitrophenylacetate (PNPA) and 0-2 ug CA1. Incubatefor 30 minutes at room temperature.Absorbance is measured at 405 nm.
Avoid freeze/thaw cycles.
GenBank
NM_001128829
Mw(kda)
29.7 kDa
Synonyms
carbonic anhydrase I, CA1
Uniprot
P00915
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Lowe, N., et al., Gene 1990 Sep 14;93(2):277-83. 2. Jover, T., et al., J Neurosci. 2002 Mar 15;22(6):2115-24.