Human BRAF (GenBank accession # NM_004333), a.a. 432-725 with N-terminal His-tag, MW=36 kDa, and p50 (GenBank accession # NM_007065), a.a. 2-378(end) with N-terminal FLAG-tag, MW=45 kDa, coexpressed in a Baculovirus infected Sf9 cell expression system. This protein exists in a complex with Cdc37 and p50.
host species: Sf9 cells
Host species: Sf9 cells
Formulation
45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 10% glycerol, and 3 mM DTT.
Species
Human
Application
Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes
Avoid freeze/thaw cycles.
GenBank
NM_004333
Mw(kda)
36 kDa
Synonyms
B-RAF1, BRAF, RAFB1, cdc37, p50
Uniprot
P15056
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775-1780. 2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.