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Home / Proteins & Peptide

BRAF, His-Tag (High Expression)

SKU : BHP18200300

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BPS Bioscience

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Mfr.No.	41237
Immunogen Region	432-725
Tag	N-terminal His-tag (BRAF)
Description	Human BRAF, also known as B-RAF1 and RAFB1, with 18 mutations to improve expression; S446A, S447A, I543A, I544S, I551K, Q562R, L588N, K630S, F667E, Y672S, A688R, L706S, Q709R, S713E, L716E, S720E, P722S, K723G. GenBank Accession #NM_004333, a.a. 444-723 with N-terminal His-tag, expressed in E. coli cells. MW = 33 kDa. Host cell line: E. coli
Formulation	40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.04% Tween20, 20% glycerol, and 350 mM imidazole.
Species	Human
Application	Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling
Notes	Avoid freeze/thaw cycles.
GenBank	NM_004333
Mw(kda)	33 kDa

Synonyms	B-RAF1, BRAF, RAFB1, cdc37, p50
Uniprot	P15056

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 12 months from date of receipt at -80?C. Avoid freeze/thaw cycles. Storing diluted enzyme is not recommended, if necessary use carrier protein (BSA 0.1 - 0.5%).

Reference	1. Sithanandam G., et al. (1990). Oncogene. 5(12): 1775-1780. 2. Shepherd C., et al. (2010). Curr. Oncol. Rep. 12(3):146-152.

Product Manual	Datasheet

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SKU	Supplier No.	Size	Your price	Quantity
BHP18200300	41237	10 ug	$412.50		Add to Cart

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