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Home / Proteins & Peptide

Bradykinin

SKU : BHP21300051

A Ligand of the B2 Bradykinin G-Protein Coupled Receptor

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Mfr.No.	GPB-200, GPB-200_1 mg, GPB-200_10 mg, GPB-200_100 mg, GPB-200_25 mg, GPB-200_5 mg, GPB-200_50 mg, GPB-200_500 mg
Description	A Ligand of the B2 Bradykinin G-Protein Coupled Receptor
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Molecular Formula	C50H73N15O11
Background	Bradykinin is a potent effector peptide that binds the bradykinin receptors B2. Bradykinin reduces blood pressure and increases vascular permeability by inducing smooth muscle relaxation and blood vessel dilation1.Bradykinin is one the most potent known factors inducing pain substances that acts on afferent sensory neurons Bradykinin stimulates the synthesis of prostaglandins2 and is a major contributor to the innate inflammatory response3. Injection of Bradykinin into the skin produces all the inflammation basic signs4. Bradykinin has been implicated also in various shock syndromes5. Application of Bradykinin to the central nervous system appears to initiate events leading to neural tissue damage as well as long lasting disturbances affecting blood-brain barrier function6.
Biological Activity	Bradykinin is an endothelial dependent vasodilator and hypotensive agent1.
Formulation	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Molecular Weight	1060.23 Da
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Source	Synthetic peptide
Concentration	30 - 300 nM
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: no Is Toxin: No
Cas Number	58-82-2
UNSPSC Code	12352202
Lead Time	1-2 Business Days

Synonyms	Kallidin I
Gene Id	BDKRB2
Sequence	RPPGFSPFR-OH
Target	B2 bradykinin receptor

Country Of Origin	Israel/IL
Purity	>=98% (HPLC)
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Applications	Calcium imaging assay

Reference	Bhoola, K.D. et al. (1992) Pharmacol. Rev. 44, 1. Brechter, A.B. and Lerner, U. H. (2007) Arthritis Rheum. 56, 910. Joseph, K. and Kaplan, A. P. 2005 Adv. Immunol. 86, 159. Marceau, F. et al. (1983) Gen. Pharmacol. 14, 209. Shin, Y. H. et al. (1996) Immunopharmacology 33, 369. Walker, K. et al. (1995) Neurochem. Int. 26, 1. Bathon, J. M. and Proud, D. (1991) Annu. Rev. Pharmacol. Toxicol. 31, 129.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300051-1	GPB-200_1 mg	1 mg	$37		Add to Cart
BHP21300051-2	GPB-200_10 mg	10 mg	$75		Add to Cart
BHP21300051-3	GPB-200_100 mg	100 mg	$188		Add to Cart
BHP21300051-4	GPB-200_25 mg	25 mg	$111		Add to Cart
BHP21300051-5	GPB-200_5 mg	5 mg	$55		Add to Cart
BHP21300051-6	GPB-200_50 mg	50 mg	$149		Add to Cart
BHP21300051-7	GPB-200_500 mg	500 mg	$705		Add to Cart

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