Human secreted B7 homolog 2 (B7-H2)-Fc fusion protein, also known as CD275, ICOSL, ICOSLG, and B7-related protein (B7RP), GenBank Accession No. NM_001283050, a.a 19-258 fused to human IgG1 Fc region (a.a. 100-330) and expressed in a HEK293 cell expression system. MW = 53.3 kDa (monomer). This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
19-258
Tag
C-terminal Fc fusion (Human IgG1)
Formulation
40 mM Tris-HCl, pH 8.0.110 mM NaCl, 2.2 mM KCl and 20% glycerol
Species
Human
Application
Useful for the study of protein binding interactions, screening inhibitors, and selectivity profiling.
Notes
Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank
NM_001283050
Mw(kda)
53.3 kDa
Synonyms
B7 homolog 2, CD275, ICOSL, ICOSLG, B7-related protein (B7RP)
Uniprot
O75144
Shipping Temperature
-80°C (dry ice)
Format
Aqueous buffer solution
Storage
At least 6 months at -80°C.
Reference
1. Guillonneau, C., et al., Transplantation. 2005; 80(4): 546-554. 2. Fan, X., et al., J. Exp. Med. 2014; 211(4): 715-725.