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Home / Proteins & Peptide

B7-1 (CD80), Fc fusion, Biotin-labeled (Human) HiP

SKU : BHP18201549

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BPS Bioscience

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Mfr.No.	71114-3
Immunogen Region	35-242
Tag	C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag
Description	Human secreted B7-1-Fc fusion protein, also known as CD80, with C-terminal Avi-tag. GenBank Accession No. NM_005191, a.a 35-242 expressed in a HEK293 cell expression system and enzymatically biotin-labeled using Avi-tag technology. MW = 52.5 kDa (monomer). This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
Formulation	8 mM Phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
Species	Human
Application	Useful for the study of protein-protein interaction and in vitro and in vivo protein function studies.
Notes	Reaction done according to CTLA4:B7-1(biotin) assay kit (Catalog # 72009) protocol. Buffer is PBS + 0.1% BSA. Blocking buffer is Superblock + 0.05% Tween-20. Coat protein was added ON at 4 deg 100 ng/well. Various concentrations of biotinylated ligand (0-8ng/ul, 0.5 ng/ul is the recommended kit condition) added for 2h at RT. Strep-HRP added for 1h at RT. HiP indicates a high purity protein with>=90% purity as measured by gel filtration. Avoid freeze/thaw cycles. Protein may be diluted to>=100 ug/ml in PBS + glycerol and stored at -80°C.
GenBank	NM_005191
Mw(kda)	52.5 kDa

Synonyms	CTLA-4 Counter-Receptor B7.1
Uniprot	P33681

Shipping Temperature	-80°C (dry ice)
Format	Aqueous buffer solution
Storage	At least 6 months at -80°C.

Reference	1. Byrum, J.N., Van Komen, J.S., Rodgers, W., J. Immunol. 2013 Sep 15;191(6):3073-3081.2. Legat, A., et al., Front Immunol. 2013 Dec 19;4:455.

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SKU	Supplier No.	Size	Your price	Quantity
BHP18201549	71114-3	500 ug	$4125		Add to Cart

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