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Home / Proteins & Peptide

Angiotensin II acetate

SKU : BHP21300110

A Potent Agonist of Angiotensin II Receptors

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Mfr.No.	SPA-165, SPA-165_10 mg, SPA-165_100 mg, SPA-165_25 mg, SPA-165_250 mg, SPA-165_5 mg, SPA-165_50 mg
Description	A Potent Agonist of Angiotensin II Receptors
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Molecular Formula	C50H71N13O12
Background	Angiotensin II (Ang II) is a natural ligand and a potent angiotensin type 1 and 2 (AT2R and AT1R) receptor agonist. It is important for maintaining normal regulation of cardiovascular function. Ang II is a primary effector peptide of the renin-angiotensin system (RAS) in a variety of tissues and is a major target for blood pressure lowering drugs. Ang II is also responsible for the release of catecholamines from the adrenal medulla and from dopaminergic neurons in the central nervous system1.Angiotensin I is metabolized to the vasoconstricting octapeptide angiotensin II by a circulating converting enzyme (ACE) by removal of two C-terminal residues.Several studies have suggested that increased levels of Ang II in the blood flow may cause endothelial cell dysfunction during the pathogenesis of conditions such as atherosclerosis and hypertension2.
Biological Activity	An Angiotensin II Type 1 and Type 2 Receptor Agonist1.
Formulation	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Molecular Weight	1046 Da
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Source	Synthetic peptide
Concentration	1 - 300 nM
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: no Is Toxin: No
Cas Number	68521-88-0
UNSPSC Code	12352202
Lead Time	1-2 Business Days

Gene Id	AGTR1, AGTR2
Sequence	DRVYIHPF-OH
Target	Angiotensin II receptors

Country Of Origin	Israel/IL
Purity	>=98% (HPLC)
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Applications	Aequorin functional assay, Calcium imaging assay

Reference	Brasch, H. et al. (1993) Hypertension 22, 699. Shi, H.X. et al. (2014) Evid. Based Complement. Alternat. Med. 2014, 682041. Watanabe, T. et al. (2005) Hypertension 45, 163.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300110-1	SPA-165_10 mg	10 mg	$85		Add to Cart
BHP21300110-2	SPA-165_100 mg	100 mg	$366		Add to Cart
BHP21300110-3	SPA-165_25 mg	25 mg	$117		Add to Cart
BHP21300110-4	SPA-165_250 mg	250 mg	$723		Add to Cart
BHP21300110-5	SPA-165_5 mg	5 mg	$66		Add to Cart
BHP21300110-6	SPA-165_50 mg	50 mg	$202		Add to Cart

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