Human Angiotensin converting enzyme 2 (ACE2) also known ACEH, GenBank Accession No. NP_068576.1, a.a. 18-740, with a C-terminal His-tag, Eu-labeled, expressed in a HEK293 expression system. MW=85 kDa. This protein runs at a higher MW by SDS-PAGE due to glycosylation.
Immunogen Region
18-740
Tag
C-terminal His-tag
Formulation
8 mM phosphate, 110 mM NaCl, 2.2 mM KCl, 20% glycerol.
Species
Human
Application
As an exopeptidase, ACE2 is useful for the study of enzyme kinetics and to screen for inhibitors. As a cell receptor for coronaviruses infection, including 2019-nCoV, ACE2 is useful for the study of protein-protein interactions.
Notes
Activity was determined using the Spike S1-Biotin (SARS-CoV-2): ACE2 TR-FRET Assay Kit (Biohippo #79949). The ACE2 antibody was incubated with biotinylated Spike S1 (Biohippo #100679), Eu-labeled ACE2 (Biohippo #100705), and the dye-labeled acceptor for one hour. Then the TR-FRET signal was measured using a fluorescence reader.