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Home / Proteins & Peptide / Proteins

Tetanus Toxin

SKU : BHP21300312

An Inhibitor of Neurotransmitter Release

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Mfr.No.	T-150, T-150_10 mcg
Description	An Inhibitor of Neurotransmitter Release
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. Soluble in any conventional buffer containing 0.01 KH2PO4, 0.1% gelatin and/or 0.1% bovine serum albumin (BSA). The use of gelatin or 0.01% BSA coated/treated glassware is recommended. Centrifuge all product preparations before use (10000 x g 5 min).
Background	Tetanus toxin is a 150 kD protein produced by the gram positive bacteria Clostridium tetani under anaerobic condition1. Tetanus toxin is a hetrodimer of A-chain (50 kD) and B-chain (100 kD) linked by one disulfide bond2,3. The A-chain has a zinc endopeptidase activity while the B-chain has a role in toxin anchoring to neuronal membranes4.Tetanus toxin spreads in the body through the lymphatic and vascular systems to the neuromuscular junction, internalized to peripheral neuron synapses, and moves by retrograde transport up the axon into the spinal cord where it can move between postsynaptic and presynaptic neurons to the CNS5. There, it inhibits neurotransmitter release by the specific degradation of the vesicle-secretory protein-Synaptobrevin6.
Biological Activity	Tetanus Toxin inhibits neurotransmitter release by cleaving synaptobrevin-2 in a zinc-dependent manner1,2.
Formulation	Lyophilized Powder.
Molecular Weight	150 kDa
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in any conventional buffer containing 0.01 KH2PO4, 0.1% gelatin and/or 0.1% bovine serum albumin (BSA). The use of gelatin or 0.01% BSA coated/treated glassware is recommended. Centrifuge all product preparations before use (10000 x g 5 min).
Source	Natural protein
Accession Number	P04958
Concentration	1 - 100 nM
Form	Lyophilized
Notes	Steril endotoxin free: no Cited Application: Electrophysiology Is Toxin: Yes
Cas Number	676570-37-9
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Modifications	Disulfide bonds between Cys439-Cys467 and Cys1077-Cys1093
Origin	Clostridium tetani

Synonyms	Tentoxylysin
Gene Id	VAMP2

Country Of Origin	Israel/IL
Purity	>=97% (HPLC)
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Reference	Laemmli, V.K. (1970) Nature 227, 680. T. M. Cook, et al. (2001) Br. J. Anaesth. 87, 477. Niemann, H. (1991) in A Sourcebook of Bacterial Protein Toxins (Alouf J. E., Freer J. H. eds), Academic Press, London 303. Lalli, G. et al. (2003) J. Cell Sci. 116, 4639. Schiavo, G. et al. (1992). Nature 359, 832. Bittner, M.A. and Holz, R.W. (1988) Neurochem. 51, 451. Matsuda, M. and Yoneda, M. (1975) Infect. Immun. 12, 1147. Schiavo, G. et al. (1992) Nature 359, 832. Schiavo, G. et al. (1992) EMBO J. 11, 3577.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300312-1	T-150_10 mcg	10 mcg	$932		Add to Cart
BHP21300312-2	T-150_10 mcg	5x10 mcg	$3711		Add to Cart

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