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Home / Proteins & Peptide / Proteins

Recombinant human TNF-alpha protein

SKU : BHP21300311

Human Tumor Necrosis Factor alpha, Recombinant, E.coli

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Mfr.No.	T-100, T-100_0.1 mg, T-100_0.25 mg, T-100_0.5 mg, T-100_1 mg, T-100_10 mcg, T-100_2 mg, T-100_25 mcg, T-100_50 mcg
Description	Human Tumor Necrosis Factor alpha, Recombinant, E.coli
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeated freeze-thaw cycles may result in loss of activity.
Molecular Formula	C778H1225N215O231S2
Background	TNF-alpha is a cytokine that binds to TNFR-55 and TNFR-75 receptors which are expressed on all somatic cells. It is derived from several types of cells but especially by activated monocytes1-2 and can induce cell death of certain tumor cell lines3. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Under certain conditions it can stimulate cell proliferation and induce cell differentiation.The secretion of the acute phase protein TNF-alpha initiates a cascade of cytokines and increases vascular permeability, thereby recruiting macrophages and neutrophils to a site of bacterial, fungal, viral or parasitic infection. Without TNF-alpha, mice infected with gram negative bacteria experience septic shock4.The cytokine possesses both growth stimulating properties and growth inhibitory processes, and it appears to have self-regulatory properties as well. For instance, TNF-alpha induces neutrophil proliferation during inflammation, but it also induces neutrophil apoptosis upon binding to the TNF-R55 receptor5.TNF-alpha participates in both inflammatory disorders of inflammatory and non-inflammatory origin6. Originally, sepsis was believed to result directly from the invading bacteria itself, but it was later recognized that host system proteins, such as TNF-alpha induced sepsis in response.TNF-alpha seems to serve as a mediator in various pathologies. A few such examples include: septic shock, cancer, AIDS, transplantation rejection, multiple sclerosis, diabetes, rheumatoid arthritis, trauma, malaria, meningitis, ischemia-reperfusion injury, adult respiratory distress syndrome, ankylosing spondylitis, inflammatory bowel disease, psoriasis, hidradenitis suppurativa and refractory asthma. Since TNF-alpha plays a role in several diseases, a substantial amount of research has been conducted concerning TNF-alpha and anti-TNF-alpha therapies. TNF-alpha inhibition can be achieved with a monoclonal antibody or with a circulating receptor fusion protein. Clinical experience so far concludes that it is safe to give TNF antagonists to cancer patients since TNF antagonist treatment results in a period of disease stabilization or better in 20% of patients with advanced cancer7-10.
Biological Activity	TNF-alpha plays a role in antitumor activity, inflammation, immune modulation, viral replication, septic shock, anorexia, cachexia, and hematopoiesis1.
Formulation	Lyophilized from filtrated Ammonium acetate solution. May contain acetate as a residual counter ion.
Molecular Weight	17.35 kDa
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
Source	Recombinant, E. coli
Accession Number	P01375
Concentration	EC50 = 1.4 pM
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: yes Is Toxin: No
Cas Number	94948-59-1
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Modifications	Disulfide bonds between: Cys145- Cys177

Synonyms	Soluble form of Tumor Necrosis Factor alpha, Cachectin
Gene Id	TNFRSF1A, TNFRSF1B
Sequence	VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL-OH
Target	TNF-R55 (TNFR1), TNF-R75 (TNFR2)

Country Of Origin	Israel/IL
Purity	>=98% (HPLC)
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Applications	Cell survival assay

Reference	Pennica, D. et al. (1984) Nature 312, 724. Aggarwal, B.B. et al. (1985) J. Biol. Chem. 260, 2345. Carswell, E.A. et al. (1975) Proc. Natl. Acad. Sci. U.S.A. 72, 3666. Janeway, C. et al. (1999) Immunobiology, 5^thedition. New York, N.Y: Garland Publishers. Murray, J. et al. (1997) Blood 90, 2772. Strieter, R. et al. (1993) Critical Care Medicine 21, S447. Madhusudan, S. et al. (2004) Clin. Cancer Res. 10, 6528. Madhusudan, S. et al. (2005) J. Clin. Oncol. 23, 5950. Harrison, M.L. et al. (2007) J. Clin. Oncol. 25, 4542. Brown, E.R. et al. (2008) Ann. Oncol. 19, 1340. Ware, C. et al. (1998) Thomson, A.W. ed. The Cytokine Handbook 3rd ed., San Diego, CA, 549.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300311-1	T-100_0.1 mg	0.1 mg	$202		Add to Cart
BHP21300311-2	T-100_0.25 mg	0.25 mg	$451		Add to Cart
BHP21300311-3	T-100_0.5 mg	0.5 mg	$808		Add to Cart
BHP21300311-4	T-100_1 mg	1 mg	$1455		Add to Cart
BHP21300311-5	T-100_10 mcg	10 mcg	$66		Add to Cart
BHP21300311-6	T-100_2 mg	2 mg	$2323		Add to Cart
BHP21300311-7	T-100_25 mcg	25 mcg	$85		Add to Cart
BHP21300311-8	T-100_50 mcg	50 mcg	$117		Add to Cart

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