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Home / Proteins & Peptide / Proteins

Recombinant human Neurotrophin-3 (NT-3) protein

SKU : BHP21300081

Human Neuotrophin-3, Recombinant, E. coli

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Mfr.No.	N-260, N-260_0.1 mg, N-260_0.25 mg, N-260_0.5 mg, N-260_10 mcg, N-260_25 mcg, N-260_5 mcg, N-260_50 mcg
Description	Human Neuotrophin-3, Recombinant, E. coli
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeated freeze-thaw cycles may result in loss of activity.
Background	The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1Of all the neurotrophins, Neurotrophin-3 (NT-3) shows the highest expression levels during the perinatal development, with most prominent expression levels in the hippocampus, the neocortex and the cerebelluM2 Release of endogenous NT-3 was observed from cultures of embryonic hippocampus following high K+ induced depolarization or stimulation with activity dependent neurotrophic factor (ADNF).3The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.4 The rat and human forms of NT-3 are 96% homologous.NT-3 has been shown to strengthen synaptic connections to motoneurons in the neonatal rat,5 to serve as an anti-inflammatory factor to suppress microglial activation,6 to play a critical role in regulating T helper 1/T helper 2 cell balance7 and to modify potassium currents in isolated inner hair cells from guinea pig cochlea.8The biological effects of NT-3 are mediated by two receptors: TrkC, which is specific for NT-3 and p75NTR, which binds all the neurotrophins.9
Biological Activity	NT-3 is involved in many biological outcomes including strengthening synaptic connections to motoneurons1 and serving as an anti-inflammatory factor to suppress microglial activation2 among many other cellular functions. NT-3 biological effects are mediated by two receptors: TrkC, which is specific for NT-3 and p75NTR, which binds all neurotrophins3.
Formulation	Lyophilized from filtrated Ammonium acetate solution. May contain acetate as a residual counter ion.
Molecular Weight	27.2 kDa (dimer)
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. For long-term storage of diluted solutions, we recommend adding 0.1% BSA. Repeat freeze-thawing may result in loss of activity.
Source	Recombinant, E. coli
Accession Number	P20783
Concentration	1 - 10 ng/ml
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: yes Is Toxin: No
UNSPSC Code	12352202
Lead Time	1-2 Business Days

Synonyms	NT-3, HDNF, Nerve growth factor 2 (NGF-2), Neurotrophic factor
Gene Id	NTRK3, NGFR
Sequence	YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT
Target	p75NTR, TrkC receptor

Country Of Origin	Israel/IL
Purity	>=98% (HPLC)
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Applications	Neurite outgrowth assay, Western blot

Reference	Roux, P. and Barker P.A. (2002) Prog. Neurobiol. 67, 203. Zhou, X.F. and Rush, R.A. (1996) Brain Res. 643, 162. Blondel, O. et al. (2000) J. Neurosci. 20, 8012. McDonald, N.Q. et al. (1991) Nature 354, 411. Arvanian, V.L. et al. (2003) J. Neurosci. 23, 8706. Tzeng, S.F. and Huang, H.Y. (2003) J. Cell Biochem. 90, 227. Sekimoto, M. et al. (2003) Immunol. Lett. 88, 221. Kimitsuki, T. et al. (2003) Auris Nasus Larynx 30, 141. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35. Arvanian, V.L. et al. (2003) J. Neurosci. 23, 8706. Tzeng, S.F. and Huang, H.Y. (2003) J. Cell Biochem. 90, 227. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300081-1	N-260_0.1 mg	0.1 mg	$824		Add to Cart
BHP21300081-2	N-260_0.25 mg	0.25 mg	$1440		Add to Cart
BHP21300081-3	N-260_0.5 mg	0.5 mg	$2839		Add to Cart
BHP21300081-4	N-260_10 mcg	10 mcg	$151		Add to Cart
BHP21300081-5	N-260_25 mcg	25 mcg	$335		Add to Cart
BHP21300081-6	N-260_5 mcg	5 mcg	$92		Add to Cart
BHP21300081-7	N-260_50 mcg	50 mcg	$607		Add to Cart

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