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Home / Proteins & Peptide / Proteins

Native mouse NGF 2.5S protein (>95%)

SKU : BHP21300073

Native Mouse Nerve Growth Factor

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Mfr.No.	N-100, N-100_0.1 mg, N-100_0.25 mg, N-100_0.5 mg, N-100_1 mg, N-100_10 mcg, N-100_25 mcg, N-100_50 mcg
Description	Native Mouse Nerve Growth Factor
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeated freeze-thaw cycles may result in loss of activity.
Background	The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3Neurotrophin secretion increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induction of cytokines combine to increase neurotrophin production in these cells after injury.4NGF is purified in three forms: the 7S, 2.5S and beta. The 7S (130 kDa) form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two alpha, one beta and two gamma subunits. The name is derived from its sedimentation co-efficient, 7S.The biologically active subunit is the beta, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the beta form because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as Platelet-derived growth factor.7 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.NGF has been shown to regulate neuronal survival, development, function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11 The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins.12
Biological Activity	NGF is purified in three forms: the 7S, 2.5S and beta. The 2.5S form is 9 amino acids shorter than the beta form because of proteolysis that occurs during the purification process1. NGF has been shown to regulate neuronal survival, development, function and plasticity2. The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins3.
Formulation	Lyophilized in 0.2% Acetic acid. May contain acetate as a residual counter ion.
Molecular Weight	26 kDa
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
Source	Natural protein
Concentration	EC50 = 0.7 nM
Form	Lyophilized
Notes	Bioassay Tested: yes Is Toxin: No
Cas Number	93928-24-6
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Origin	Native protein isolated from mouse submaxillary glands.

Synonyms	Nerve Growth Factor 2.5S
Gene Id	NGFR, NTRK1
Target	p75NTR, TrkA receptors

Country Of Origin	Israel/IL
Purity	>95% (HPLC)
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Applications	Neurite outgrowth assay, Cell survival assay

Reference	Roux, P.P. and Barker P.A. (2002) Prog. Neurobiol. 67, 203. Levi-Montalcini, R. (1966) Harvey Lect. 60, 217. Farinas, I. et al. (1998) Neuron 21, 325. Levi-Montalcini, R. et al. (1996) Trends Neurosci. 19, 514. Bradshaw, R.A. (1978) Annu. Rev. Biochem. 47, 191. Bocchini, V. and Angeletti, P.U. (1969) Proc. Natl. Acad. Sci. U.S.A. 64, 787. McDonald, N.Q. et al. (1991) Nature 354, 411. Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677. Freund V. and Frossard, N. (1994) Prog. Brain Res. 146, 335. Raychaudhuri, S.P. and Raychaudhuri, S.K. (2004) Prog. Brain Res. 146, 433. Kawamoto, K. and Matsuda, H. (2004) Prog. Brain Res. 146, 369. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35. Darling, T. and Shooter, E.M. (1984) "Methods for preparation and assay of nerve growth factor", Cell Culture Methods for Molecular and Cellular Biology, vol. 4 (Eds. D. W. Barnes, D.A. Sinbasku, and G.H. Sato), pp. 79-83, Alan R. Liss, New York. Bocchini, V. and Angeletti, P.U. (1969) Proc. Natl. Acad. Sci. U.S.A. 64, 787. Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300073-1	N-100_0.1 mg	0.1 mg	$361		Add to Cart
BHP21300073-2	N-100_0.25 mg	0.25 mg	$824		Add to Cart
BHP21300073-3	N-100_0.5 mg	0.5 mg	$921		Add to Cart
BHP21300073-4	N-100_1 mg	1 mg	$1018		Add to Cart
BHP21300073-5	N-100_10 mcg	10 mcg	$92		Add to Cart
BHP21300073-6	N-100_25 mcg	25 mcg	$182		Add to Cart
BHP21300073-7	N-100_50 mcg	50 mcg	$286		Add to Cart

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