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Home / Proteins & Peptide / Proteins

mouse NGF 2.5S-Biotin

SKU : BHP21300076

Biotin Labeled Mouse Nerve Growth Factor

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Mfr.No.	N-240-B, N-240-B_2 mcg
Description	Biotin Labeled Mouse Nerve Growth Factor
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (5 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Background	The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic protein growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS1.NGF, the first member of the family to be discovered, was originally purified as a factor supporting and regulating survival, development, function and plasticity of sympathetic and sensory spinal neurons in central and peripheral nervous systems in vivo as well as in vitro2-4. It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target5.Neurotrophin secretion increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however, direct trauma and induction of cytokines combine to increase neurotrophin production in these cells after injury6.NGF is purified in three forms: the 7S, 2.5S and beta, in which the biologically active subunit is the beta subunit. The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as Platelet-derived growth factor7.Additionally, the involvement of NGF was recently discovered in processes such as asthma8, psoriasis9 and wound healing10. The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins11.For the past three decades, biotinylated derivatives of NGF have been widely used in the literature as useful probes for the study of the binding and the initial intracellular processing of this growth factor by cells bearing TrkA NGF receptor12. First studies using 125I-NGF have visualized the intracellular location of NGF12. A biotinylated derivative of NGF that retains biological activity, in conjunction with the appropriate avidin conjugates and fluorescent or confocal microscopic techniques, improve the sensitivity and resolution in many applications. Some examples of applications are: targeting of liposomes containing biotinylated NGF to cells bearing TrkA NGF receptors13; elucidating the kinetics and route of ligand-induced internalization of the p75 receptor through signaling endosomes in cycling and differentiated PC12 cells using biotinylated derivative of NGF14; electrically controlled NGF-Biotin delivery from biotin-doped conductive polymer15; studying the retrograde axonal transport mechanism of NGF signals from the axon terminal to the cell body16,17.
Biological Activity	NGF is purified in three forms: the 7S, 2.5S and beta. The 2.5S form is 9 amino acids shorter than the beta form because of proteolysis that occurs during the purification process1. NGF has been shown to regulate neuronal survival, development, function and plasticity2. The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins3.
Formulation	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Molecular Weight	~26 kDa
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (50 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Source	Modified natural protein
Concentration	EC50 = 0.5 nM
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: no Is Toxin: No
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Modifications	LC-Biotin
Origin	Native protein isolated from mouse submaxillary glands.

Synonyms	Nerve Growth Factor 2.5S
Gene Id	NGFR, NTRK1
Target	p75NTR, TrkA receptors

Country Of Origin	Israel/IL
Storage	Storage After Reconstitution: Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day.

Applications	Neurite outgrowth assay, Cell survival assay, Live cell imaging, Immunofluorescence, Fluorescence staining

Reference	Roux, P.P. and Barker P.A. (2002) Prog. Neurobiol. 67, 203. Levi-Montalcini, R. (1966) Harvey Lect. 60, 217. Kalb, R. (2005) Trends Neurosci. 28, 5. Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677. Farinas, I. et al. (1998) Neuron 21, 325. Levi-Montalcini, R. et al. (1996) Trends Neurosci. 19, 514. McDonald, N.Q. et al. (1991) Nature 354, 411. Freund V. and Frossard, N. (1994) Prog. Brain Res. 146, 335. Raychaudhuri, S.P. and Raychaudhuri, S.K. (2004) Prog. Brain Res. 146, 433. Kawamoto, K. and Matsuda, H. (2004) Prog. Brain Res. 146, 369. Rosenberg, M.B. et al. (1986) J. Neurochem. 46, 641. Rosenberg, M.B. et al. (1987) J. Neurochem. 48, 865. Bronfman, F.C. et al. (2003) J. Neurosci. 23, 3209. George, P.M. et al. (2006) Adv. Materials 18, 577. Cui, B. et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104, 13666. Perlson, E. et al. (2009) J. Neurosci. 29, 9903. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35. Bocchini, V. and Angeletti, P.U. (1969) Proc. Natl. Acad. Sci. U.S.A. 64, 787. Huang, E.J. and Reichardt, L.F. (2001) Annu. Rev. Neurosci. 24, 677. Teng, K.K. and Hempstead, B.L. (2004) Cell Mol. Life Sci. 61, 35.

Product Manual	SDS

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300076-1	N-240-B_2 mcg	2 mcg	$1008		Add to Cart
BHP21300076-2	N-240-B_2 mcg	5x2 mcg	$4024		Add to Cart

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