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Home / Proteins & Peptide / Proteins

Human TrkC-Fc Chimera

SKU : BHP21300100

An Inhibitor of NT-3 Activity

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Mfr.No.	RPC-004, RPC-004_5 mcg, RPC-004_50 mcg
Description	An Inhibitor of NT-3 Activity
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeated freeze-thaw cycles may result in loss of activity.
Background	TrkC-Fc chimera is a recombinant protein, a soluble fusion of the TrKC receptor which comprises the extracellular domains of the human TrkC fused to the IgG heavy chain. TrkC-Fc has the binding specificity and affinity of TrkC receptor that can block the biological activity of neurotrophin-31.The neurotrophin family includes four members- nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These are encoded by NGF, BDNF, NTF3, and NTF4 human genes, respectively2.Neurotrophins activate in part Trk family receptor protein-Tropomyosin related kinases. The Trk family includes three members: TrkA, TrkB, and TrkC, encoded by NTRK1, NTRK2, and NTRK3 human genes, respectively. TrkC has the highest affinity for NT-32.The Trk structure includes a cytoplasmic domain containing several sites of tyrosine phosphorylation which are responsible for activating intracellular signaling cascades. The extracellular domain consists of a cysteine-rich cluster followed by three leucine-rich repeats, another cysteine-rich cluster and two immunoglobulin-like domains3,4.
Biological Activity	Human TrkC-Fc Chimera inhibits NT-3-induced phosphorylation of ERK1/2 in HEK293 cells expressing TrkB receptors.
Formulation	Lyophilized in PBS.
Molecular Weight	~100 kDa
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
Source	Recombinant, HEK 293-6E cells.
Concentration	0.2 - 0.5 ug/ml in the prescence of 30 ng/ml of human NT-3.
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: yes Is Toxin: No
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Modifications	Glycosylation

Gene Id	NTF3
Sequence	Human TrkC (Cys32-Asp428)-DIEGEMD-Human IgG1 (Pro100-Lys330)
Target	NT-3

Country Of Origin	Israel/IL
Purity	>90% (HPLC)
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Applications	Western blot

Reference	Ramos, B. et al. (2009) Mol. Cell. Neurosci. 42, 152. Green, S.H. et al. (2012) Anat. Rec. 295, 1877. Huang, E.J. et al. (2003) Annu. Rev. Biochem. 72, 609. Reichardt, L.F. (2006) Philos. Trans. R. Soc. Lond. B Biol. Sci. 361, 1545. Bouzas-Rodriguez, J. et al. (2010) J. Clin. Invest. 120, 850. Palazzo, E. et al. (2012) J. Cell. Physiol. 227, 1017. Ramos, B. et al. (2009) Mol. Cell. Neurosci. 42, 152.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300100-1	RPC-004_5 mcg	5 mcg	$170		Add to Cart
BHP21300100-2	RPC-004_50 mcg	50 mcg	$561		Add to Cart
BHP21300100-3	RPC-004_5 mcg	5x5 mcg	$670		Add to Cart

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