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Home / Proteins & Peptide / Proteins

Human TrkB-Fc Chimera

SKU : BHP21300098

An Inhibitor of BDNF Activity

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Mfr.No.	RPC-001, RPC-001_5 mcg, RPC-001_50 mcg
Description	An Inhibitor of BDNF Activity
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeated freeze-thaw cycles may result in loss of activity.
Background	TrkB is a receptor tyrosine kinase of the Trk family. It is activated by brain-derived neurotrophic factor (BDNF), neurotrophin-3, -4 and -5 and is involved in the development and maintenance of the nervous system1. TrkB-Fc is a fusion protein combining the extracellular binding domain of TrkB and the Fc domain of human IgG. TrkB-Fc is a tool for studying the biological actions of BDNF 2.A large number of in vitro studies support the notion that TrkB-Fc inhibits BDNF activity3. Addition of TrkB-Fc to hippocampal and cortical slices and cultured cortical, striatal, and dentate granule cells either abolishes or opposes the effect of BDNF. The TrkB-Fc fusion protein, a specific inhibitor of Trk kinase activity, K252, and a TrkB neutralizing antibody all have similar BDNF-blocking effects. In addition, administration of TrkB-Fc in vivo has consequences that are in accordance with decreased BDNF activity. Systemic nerve growth factor treatment, which leads to a condition resembling peripheral inflammation, raises BDNF levels in sensory neurons and increases nociceptive spinal reflex excitability. This increased central excitability is reduced by TrkB-Fc4. Moreover, intraventricular delivery of TrkB-Fc suppresses epileptogenesis, similar to what has been observed in heterozygous BDNF knockout mice and in transgenic mice overexpressing truncated TrkB receptors and with decreased endogenous BDNF levels5. In contrast to these data, Croll et al.2 reported that TrkB-Fc can potentiate BDNF-induced TrkB phosphorylation. However, this effect was observed only when TrkB-Fc and BDNF were coinfused intracerebrally in equimolar concentrations.
Biological Activity	TrkB-Fc Chimera inhibits BDNF-induced phosphorylation of ERK1/2 in HEK293 cells expressing TrkB receptors1-8.
Formulation	Lyophilized in PBS.
Molecular Weight	~120 kDa
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
Source	Recombinant, HEK 293-6E cells.
Concentration	0.1 - 0.4 ug/ml in the presence of 10 ng/ml of human BDNF.
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: yes Is Toxin: No
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Modifications	Glycosylation

Gene Id	BDNF
Sequence	Human TrkB (Cys32-His430)-DIEGEMD-Human IgG1 (Pro100-Lys330)
Target	BDNF

Country Of Origin	Israel/IL
Purity	>90% (HPLC)
Storage	Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.

Applications	Western blot

Reference	Klein, R. et al. (1990) Cell 61, 647. Croll, S.D. et al. (1998) Exp. Neurol. 152, 20. Shelton, D.L. et al. (1995) J. Neurosci. 15, 477. Gustafsson, E. et al. (2003) Stroke 34, 2710. Lahteinen, S. et al. (2002) Eur J Neurosci. 15, 721. Quadrato, G. et al. (2012) Proc. Natl. Acad. Sci. U.S.A. 109, E1499. Boulle, F. et al. (2012) Prog. Neurobiol. 98, 197. Duclot, F. and Kabbaj, M. (2013) J. Neurosci. 33, 11048. Cabelli, R.J. et al. (1997) Neuron 19, 63. Kang, H. et al. (1997) Neuron 19, 653. McAllister, A.K. et al. (1997) Neuron 18, 767. Croll, S.D. et al. (1998) Exp. Neurol. 152, 20. Zhang, X. et al. (2012) J. Neural. Transm. 119, 329.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300098-1	RPC-001_5 mcg	5 mcg	$170		Add to Cart
BHP21300098-2	RPC-001_50 mcg	50 mcg	$561		Add to Cart
BHP21300098-3	RPC-001_5 mcg	5x5 mcg	$670		Add to Cart

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