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Home / Proteins & Peptide / Proteins

Apamin-Biotin

SKU : BHP21300128

A Blocker of Small Conductance Ca2+-Activated K+ Channels (SK Type) Conjugated to Biotin

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Mfr.No.	STA-200-B, STA-200-B_10 mcg
Description	A Blocker of Small Conductance Ca2+-Activated K+ Channels (SK Type) Conjugated to Biotin
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (5 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Background	Apamin is a natural peptide isolated and purified from Apis mellifera bee venoM Apamin blocks small conductance Ca2+-activated K+ channels (SK). It is specific for the SK1-3 isoforms, but ineffective in blocking other calcium activated K+ channels2. In mammalian cell lines, Apamin blocks expressed hSK1 with an IC50 of ~10 nM3, rSK1 and rSK2 with IC50 of ~3 and
Biological Activity	Apamin is a blocker of voltage-independent Ca2+-activated K+ (SK) channels1.
Formulation	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Molecular Weight	2367 Da
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water to high-micromolar concentrations (50 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
Source	Modified synthetic peptide
Concentration	1 - 50 nM
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: no Cited Application: Electrophysiology Is Toxin: Yes
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Modifications	Disulfide bonds between: Cys1-Cys11, and Cys3-Cys15 His18 - C-terminal amidation LC-Biotin
Origin	Apis mellifera (Honeybee)

Synonyms	APM, Apamine
Gene Id	KCNN1,KCNN2,KCNN3
Sequence	Biotin-CNCKAPETALCARRCQQH-NH2
Target	KCa2 K+ channels

Country Of Origin	Israel/IL
Storage	Storage After Reconstitution: Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: Store the reconstituted solution for the shortest time possible at -20°C. Avoid multiple freeze- thaw cycles. We do not recommend storing the toxin in working solutions for longer than a day.

Applications	Electrophysiology, Immunofluorescence

Reference	Gauldie, J. et al. (1976) Eur. J. Biochem. 61, 369. Logsdon, N.J. et al. (1997) J. Biol. Chem. 272, 32723. Shah M. and Haylett D.G. (2000) Br. J. Pharm. 129, 627. Strobaek D. et al. (2000) Br. J. Pharm. 129, 991. Barfod, E.T. et al. (2001) Am. J. Physiol. 280, C836. Desai, R. et al. (2000) J. Biol. Chem. 275, 39954. Hosseini, R. et al. (2001) J. Physiol. 535, 323. Kong, I.D. et al. (2000) Am. J. Physiol. 278, C352. Nagayama, T. et al. (2000) Am. J. Physiol. 279, R1731. Shahidi, S. et al. (1993) Biochim. Biophys. Acta 1157, 74.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300128-1	STA-200-B_10 mcg	10 mcg	$811		Add to Cart
BHP21300128-2	STA-200-B_10 mcg	5x10 mcg	$3237		Add to Cart

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