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Home / Proteins & Peptide / Proteins

alpha-Conotoxin GI-ATTO Fluor-647N

SKU : BHP21300172

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Alomone Labs

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Mfr.No.	STC-500-FRN, STC-500-FRN_10 mcg, STC-500-FRN_5 mcg
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. The product is lyophilized in 0.5 ml conical vial. The product is soluble in pure water to high-micromolar concentrations (5 uM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity. Avoid exposure to light.
Background	alpha-Conotoxin GI is a 13 amino acid peptidyl toxin isolated from the Conus geographus (Geography cone) venom1,2,3. It belongs to the Conotoxin A superfamily and reversibly blocks alpha/delta nicotinic ACh channel receptors (nAChR). alpha-Conotoxin GI reversibly inhibits the high affinity alpha/delta site on mouse muscle-derived BC3H-1 receptor, a nAChR expressed on postsynaptic membranes4,5. Other low site (alpha/gamma site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by alpha-Conotoxin GI5.
Biological Activity	alpha-Conotoxin GI inhibits alpha/delta site on mouse muscle-derived BC3H-1 nicotinic acetylcholine receptors (nAChR) expressed on postsynaptic membranes. Other site (alpha/gamma site) on nicotinic receptors from Torpedo californica electric organ is also inhibited by alpha-Conotoxin GI1,2.
Formulation	Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
Molecular Weight	2065 Da
Source	Modified synthetic peptide
Concentration	400 nM- 4 uM
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: no
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Modifications	Cys13 - C-terminal amidation ATTO-647NDisulfide bonds between: Cys2-Cys7, Cys3-Cys13
Origin	Conus geographus (Geography cone) (Nubecula geographus)

Synonyms	G1, Alpha-conotoxin GI
Gene Id	CHRNA1,CHRNB1,CHRND,CHRNG, CHRNE
Sequence	ECCNPACGRHYSC-NH2
Target	alpha1/beta1/delta/gamma nAChR

Country Of Origin	Israel/IL
Storage	Storage After Reconstitution: Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Avoid exposure to light. Storage of solutions: Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day. Avoid exposure to light.

Applications	Electrophysiology, Live cell imaging, Immunofluorescence, Fluorescence staining, Direct flow cytometry

Reference	Gray, W.R. et al. (1981) The J. Biol. Chem. 256, 4734. Nishiuchi, Y. and Sakakibara, S. (1982) FEBS LETT. 148, 260. Guddat, L.W. et al. (1996) Biochemistry 35, 11329. Groebe, D.R. et al. (1995) Mol. Pharmacol. 48, 105. Groebe, D.R. et al. (1997) Biochemistry 36, 6469. Groebe, D.R. et al. (1995) Mol. Pharmacol. 48, 105. Groebe, D.R. et al. (1997) Biochemistry 36, 6469.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300172-1	STC-500-FRN_10 mcg	10 mcg	$821		Add to Cart
BHP21300172-2	STC-500-FRN_5 mcg	5 mcg	$543		Add to Cart
BHP21300172-3	STC-500-FRN_10 mcg	2x10 mcg	$1439		Add to Cart
BHP21300172-4	STC-500-FRN_10 mcg	5x10 mcg	$2439		Add to Cart
BHP21300172-5	STC-500-FRN_10 mcg	10x10 mcg	$3345		Add to Cart

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