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Home / Proteins & Peptide / Proteins

Ala5-Galanin (2-11)

SKU : BHP21300059

A Potent and Selective Agonist of Galanin 2 Receptor

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Alomone Labs

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Mfr.No.	GPG-300, GPG-300_1 mg, GPG-300_10 mg, GPG-300_2.5 mg, GPG-300_5 mg
Description	A Potent and Selective Agonist of Galanin 2 Receptor
Reconstitution	Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. Avoid multiple freeze-thaw cycles to maintain biological activity. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended.
Molecular Formula	C54H81N13O13
Background	Ala5-Galanin (2-11) is a galanin analogue that acts as a potent, selective and specific Galanin 2 receptor (GalR2) agonist. Ala5-Galanin (2-11) is a full agonist of Gal2R with no detectable binding to human galanin receptors 1 and 3. Ala5-Galanin (2-11) is based on the short galanin fragment galanin (2-11) including a single amino acid substitution of alanine instead of serine at position five.To date, Ala5-Galanin (2-11) is the shortest galanin receptor 2 specific agonist reported1. Ala5-Galanin (2-11) activates GalR2 with an EC50 value of 1.01 ± 0.18 uM1.Galanin receptors belong to the family of G-protein coupled receptors and consist of three subtypes: GalR1, GalR2, and GalR32.
Biological Activity	Ala5-Galanin (2-11) is a Galanin 2 receptor agonist1.
Formulation	Lyophilized in 50% Acetic acid. May contain acetate as a residual counter ion.
Molecular Weight	1120 Da
Solubility	Centrifuge the vial before adding solvent (10,000 x g for 5 minutes). The lyophilizate may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. Soluble in DMSO. Prepare a concentrated stock solution by dissolving the lyophilized peptide in DMSO first (e.g., at a concentration between 100-1000x of the final working concentration). Once the peptide is completely dissolved in DMSO, slowly dilute the solution into the working buffer (or water) to the desired final working concentration. Centrifuge all product preparations before use. It is recommended to keep the DMSO concentration as low as possible. For cell assays, a final concentration of 0.1%0.5% DMSO (v/v) is considered safe. For other experiments, a 5% DMSO (v/v) concentration is recommended.
Source	Synthetic peptide
Concentration	1 - 10 nM
Form	Lyophilized
Notes	Bioassay Tested: yes Steril endotoxin free: no Is Toxin: No
UNSPSC Code	12352202
Lead Time	1-2 Business Days
Modifications	Leu10 - C-terminal amidation

Gene Id	GALR2
Sequence	WTLNAAGYLL-NH2
Target	Galanin 2 receptors

Country Of Origin	Israel/IL
Purity	>=98% (HPLC)
Storage	Storage After Reconstitution: Store the reconstituted solution for the shortest time possible at -20°C. We do not recommend storing the product in working solution for longer than one day. Avoid multiple freeze-thaw cycles. Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture. Storage of solutions: Store the reconstituted solution at -20°C for the shortest time possible. Avoid multiple freeze-thaw cycles. We do not recommend storing the product in working solutions for longer than a day.

Applications	Aequorin functional assay, Calcium imaging assay

Reference	Webling K. et al. (2016) Neuropeptides 60, 75. Liu, M. et al. (2013) PLoS One 8, e63473. Webling K. et al. (2016) Neuropeptides 60, 75.

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Combination SKU	Supplier No.	Size	Your price	Quantity
BHP21300059-1	GPG-300_1 mg	1 mg	$202		Add to Cart
BHP21300059-2	GPG-300_10 mg	10 mg	$680		Add to Cart
BHP21300059-3	GPG-300_2.5 mg	2.5 mg	$337		Add to Cart
BHP21300059-4	GPG-300_5 mg	5 mg	$470		Add to Cart

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