Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.
Short Description
Unstained / Protein Marker / Protein Standard / SDS-PAGE / Western Blot / Staining Reagent / Coomassie Blue / GeneDireX
The Unveil Unstained Protein Ladder is a mixture of12 unstained recombinant proteins covering a wide range of molecular weights from 10 to 200 kDa. The proteins resolve into clearly identifiable bands when separated on the SDS-PAGE gel (MES buffer), with the intensified 25kDa and 85kDa protein bands serving as the two reference bands. The Unveil Unstained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer and is ready to use.
Storage Buffer : 20 mM Tris-phosphate(pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.
Quality Control : Under suggested conditions, Unveil Unstained Protein Ladder resolves 12 major bands in 4-12%Bis- Tris Gel(MES buffer) and after Western blotting to the nitrocellulose membrane.
Storage : Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long-term storage, store at -20°C.
Note. The apparent molecular weight (kDa) of each protein has been determined by calibration against unstained protein standards; supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
All products are for research use only.
Caution : Not intended for human or animal diagnostic or therapeutic uses.
Time-lapse Sds-page Running Video
Lane 1: GeneDireX PM019-0500 (Bio-Helix) 5ul Lane 2: GeneDireX PM008-0500 (Bio-Helix) 5ul Lane 3: GeneDireX PM007-0500 (Bio-Helix) 5ul Lane 4: GeneDireX PM006-0500 (Bio-Helix) 5ul Lane 5: GeneDireX PM001-0500 (Bio-Helix) 5ul Lane 6: GeneDireX PM019-0500 (Bio-Helix) 5ul Lane 7: GeneDireX PM001-0500 (Bio-Helix) 5ul Lane 8: GeneDireX PMU12-0500 (Bio-Helix) 5ul Lane 9: thermo Mark12 5ul Lane 10: Bio-Rad 161-0317 5ul
*NuPAGE Bis-Tris 4-12% was used to run SDS-PAGE in MOPS and MES buffer. 5ul of protein ladder samples was loaded into each lane.
Storage Buffer
20 mM Tris-phosphate(pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.